In preclinical research radiolabeled nanoparticles have been attracting interest as a new class of imaging probes. Assuming good stability of solid lipid nanoparticles (SLNs) under physiological conditions, radiolabeled SLNs can be used for imaging and measuring uptake in target tissue. Present study was performed to evaluate biological behavior of temozolomide (TMZ) loaded solid lipid nanoparticles (SLN-TMZ) in vivo and in vitro. Lipid nanoparticles were prepared by emulsification and low-temperature solidification method. ζ potential, morphology and particle size of nanoparticles were determined. Biological behavior of 99mTc(CO)3+ radiolabeled SLN-TMZ were investigated in vitro on U87/Daoy cell lines and in vivo on female Wistar Albino rats. Obtained results of in vitro incorporation, in vivo biodistribution and gamma imaging studies on radiolabeled SLN-TMZ show that the radiolabeled solid lipid nanoparticles could have potential as a drug delivery system for TMZ.
Background:
Peptide-based agents are used in molecular imaging due to their unique properties, such as
rapid clearance from the circulation, high affinity and target selectivity. Many of the radiolabeled peptides have
been clinically experienced with diagnostic accuracy. The aim of this study was to investigate in vivo biological
behavior of [99mTc(CO)3(H2O)3]+ radiolabeled glycylglycine (GlyGly).
Methods:
Glycylglycine was radiolabeled with a high radiolabeling yield of 94.69±2%, and quality control of the
radiolabeling process was performed by thin layer radiochromatography (TLRC) and High-Performance Liquid
Radiochromatography (HPLRC). Lipophilicity study for radiolabeled complex (99mTc(CO)3-Gly-Gly) was carried
out using solvent extraction. The in vivo evaluation was performed by both biodistribution and SPECT imaging.
Results:
The high radiolabelling yield of 99mTc(CO)3-GlyGly was obtained and verified by TLRC and HPLRC as
well. According to the in vivo results, SPECT images and biodistribution data are in good accordance. The
excretion route from the body was both hepatobiliary and renal.
Conclusion:
This study shows that 99mTc(CO)3-GlyGly has the potential to be used as a peptide-based imaging
agent. Further studies, 99mTc(CO)3-GlyGly can be performed on tumor-bearing animals.
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