The hardening of in vitro raised plantlets is essential for better survival and successful establishment. In the present study an attempt was made to find out the best ex vitro condition (single shade net and double shade net) for hardening of five different clones of sugarcane. The effect of temperature, light intensity and humidity on hardening of elite sugarcane genotypes were evaluated under two different hardening conditions. The results obtained in the present investigation concluded that both the ex vitro acclimatization conditions showed almost similar effect on number of leaves per plant. However, for chlorophyll content (mg/g), root characters, viz. number of roots, root length (cm), root volume (cm3) and survival ability, double shade net was found to be optimum for all the genotypes, viz. CoVC 09-61-02, CoVC 07-06-05, CoVC 09-61-07, CoC 671 and CoVC 10-38-07. Whereas, CoVC 07-06-05 and CoC 671 produced optimum shoot length under single shade net. Hence, it is clear that the double shade net served as better hardening condition as revealed from better survival and development of appropriate morphological characters of all the sugarcane clones.
Cold tolerance during germination is important for ensuring fast and uniform crop establishment. Rice plants are injured at the seedling stage when they are grown in early spring in temperate or subtropical environments. The productivity of rice is particularly low during spring due to cold, which is an important abiotic constraint, where low temperature prevails below 18 ºC. The present study was taken up to evaluate cold tolerance in 86 rice genotypes based on seed and seedling parameters as per Cruz and Milach (2004). Seeds were germinated under two conditions i.e.,160C for 28 days (cold) and 280C for 7 days (control).The genotypes showed highly significant differences forparameters such as germination (%), germination index (%), percentage of seeds with coleoptile superior to 5 mm (PERCOL), percentage of reduction in coleoptile length (REDCOL) and coleoptile regrowth (COLREG, cm).Both REDCOL and COLREG seem to be the most adequate characteristics to be used to evaluate cold tolerance during the germination period in rice. Among those genotypes, AC 35548, JBT 37/164, PS 353, KMP-175 andThanu were adjudged as promising when screened for cold tolerance under laboratory conditions based on the aforementioned parameters.
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