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Sixty four french bean genotypes were analyzed with 15 microsatellite markers to test variation. Among the total 15 SSR primers used, 10 primers were polymorphic and 5 were monomorphic. Of the 15 SSR primers used, 10 primer pairs showed polymorphism. The number of alleles produced by different primers ranged from two to four. The high level of polymorphism (66.66%) was obtained. The cluster construction based on SSR marker data revealed significant genetic variation among the genotypes. SSR marker system showed high dissimilarity among the french bean genotypes. Cluster diagram constructed using polymorphic markers identified two major clusters. All exotic collections, landraces, local types and released varieties were grouped in same cluster with some indigenous collection, whereas cluster A comprised only of indigenous collection.

A new leaf blight disease of browntop millet (Brachiaria ramosa) was noticed during rainy season (Kharif) 2018 at small millet experimental field, University of Agricultural Sciences, Gandhi Krishi Vignana Kendra (GKVK), Bengaluru, India. To assess the disease severity, an intensive roving survey was conducted during the 2019 cropping season. Based on the morphological characterization, the causal agent of leaf blight disease was identified as Bipolaris spp. Further sequencing and combined gene analysis of ITS (internal transcribed spacer of rDNA), GAPDH (glyceraldehyde 3‐phosphate dehydrogenase) and LSU (large subunit) of all the nine isolates confirmed the pathogen as B. setariae. Pathogenicity study showed that all the isolates were pathogenic and caused leaf blight symptoms on browntop millet. The B. setariae isolates showed marked variability with respect to disease incidence on browntop millet (cv. Dundu korale) under artificial inoculation conditions. However, the host range was limited only to browntop millet and found non‐pathogenic to other six small millets examined. To our knowledge, this is the first completely described study on characterization of B. setariae causing leaf blight disease of browntop millet in India.

Sugarcane (Saccharum species hybrid) is not only an important cash crop but also major source of sugar industry worldwide. An effort was made to understand the effect of varying levels of medium pH for testing differential response of sugarcane genotypes (Co 86032, Co 85004 and Co 99004) on in vitro shoot initiation, induction, multiplication and root formation. The shoot apical meristem of the three varieties was cultured on MS medium with different pH levels. Aseptic meristem cultures of the three sugarcane genotypes were cultured in vitro on the medium with five pH concentrations, viz. 4.8, 5.0, 5.4, 5.6 and 5.8. Co 85004 was found to show its response towards the medium pH at 5.6 in producing highest number of shoots (~22). However, Co 99004 responded well towards medium pH at 5.4 which produced ~21 numbers of shoots and Co 86032 exhibited its potentiality towards pH only at 5.8 levels (~17) on 180 days after inoculation. The response of all the genotypes were on par towards pH 7.0 which produced highest number of roots. At pH 5.8 genotype, Co 86032 produced highest shoot length (~3 cm) followed by Co 85004 (~2 cm) and Co 99004 (~2 cm). The study revealed that medium adjusted with pH 4.8, pH 5.8 and pH 7.0 levels resulted in optimum in vitro shoot initiation, induction, multiplication and root formation responses in Co 85004, Co 99004 and Co 86032, respectively.

A filed experiment was conducted at ZARS, V. C. Farm, Mandya to enhance the crop and water productivity in sugarcane during 2017-18 and 2018-19. The design used was strip plot design with planting methods as a horizontal factor and irrigation scheduling as a vertical factor with three replications. Among the planting methods, 120 cm row spaced furrow planting with dhaincha green manure sowing at 30 DAP and mulching at 75 DAP recorded significantly higher cane yield (158.5 and 161.0 t/ha, respectively) as compared to others during both the years of experimentation. Among the irrigation schedules, IW/CPE ratio of 1.0 recorded significantly higher yield (168.1 and 170.8 t/ha, respectively) as compared to IW/CPE ratio of 0.60 (129.7 and 131.8 t/ha, respectively). The total water used was significantly lower in 120 cm spaced furrow planting with alternate skip furrow irrigation afte5r earthing + green manure mulching (1773 and 1751 mm, respectively) and it saved 16% of irrigation water. Scheduling of irrigation at IW/CPE ratio of 0.6 consumed lowest amount of irrigation water (1725 mm) and saved 18% of irrigation water.

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