The effect of a n enzyme preparation of Strqtomyces spec. KT3 was tested in view of its cell wall 1 ytic activity on cells of Saccharomyces cerevisiue. According to electron micrographs under the influence of the enzyme preparation protoplasts but no spheroplasts were formed. The yield of protoplasts amounted dependent on the enzyme charge to 75-99 per cent, most of the protoplasts were released after 60 minutes. Cells of 12 strains of Saccharomyces cerevisiae were able to be protoplasted and stabilized immediately thereafter by mannitol (0.8 M) or KCI (0.6 M). The presence of stabilizers already in the enzyme-containing medium inhibited, however, protoplasting considerably. The stabilized protoplasts could be stored for six days a t 4OC without any noticeable changes. EinfiihrungBiotechnologische Verfahren mit Mikroorganismen setzen leistungsstarke Stamme vokaus. Es ist deshalb eine vorrangige Aufgabe, die Leistungciparameter bestimmter Stamme zu verbecisern oder neue ProduktionsstBmme zu schaffen, die hohe Biomasse-bzw. Produktausbeuten gewahrleisten. Um dieses Ziel zu erreichen, bedient man sich verschiedener Methoden, eine davon ist die in den letzten Jahren mehrfach angewandte Methode der Genomzusammenfuhrung auf parasexuellem Wege durch induzierte Fusion von Protoplasten selektierter Stamme (u-a. FERENCZY [l], ALFOLDI [2], MACH [3]). Sehr haufig wurden zur Protoplastierung der Magendarmsaft von Helix pamatia oder daraus hergestellte Enzyme eingesetzt (EDDY u. WILLIAMSON [4], PEBERDY [5], DIA-TEWA et al. [6], WEBER [7] u. a.). Eine weitere Moglichkeit, Protoplasten zu gewinnen, ergibt sich durch Anwendung zellwandlytischer mikrobieller Enzyme (9. Kuo u. Y m -MOTO [8] sowie POPOV u. KONSTANTINOVA [9]). Wir untersuchten die Wirkung eines Streptomyceten-Enzympraparates auf die Protoplastenbildung verschiedener Stamme von Saecharomyces ceremkiae.
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