Abstract:The effect of a n enzyme preparation of Strqtomyces spec. KT3 was tested in view of its cell wall 1 ytic activity on cells of Saccharomyces cerevisiue. According to electron micrographs under the influence of the enzyme preparation protoplasts but no spheroplasts were formed. The yield of protoplasts amounted dependent on the enzyme charge to 75-99 per cent, most of the protoplasts were released after 60 minutes. Cells of 12 strains of Saccharomyces cerevisiae were able to be protoplasted and stabilized immedi… Show more
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