A bovine herpesvirus (BHV) strain designated B11-41, was recently isolated from the spinal cord of a cow with astasia. By BHV type specific polymerase chain reaction (PCR), a BHV-4 specific fragment was amplified from the DNA of strain B11-41. The nucleotide sequence of the PCR product revealed high homologies (98.4% and 99.8%) with those of two BHV-4 strains (Movar 33/63 and 86-068). The BamHI restriction endonuclease cleavage patterns of the B11-41 were more similar to those of BHV-4 than those of other types of BHV. BHV-4 is divided into two groups, European and American. The EcoRI, and BamHI, and HindIII restriction endonuclease analysis demonstrated that strain B11-41 was closely related to the American type of BHV-4. It was concluded that B11-41 was identified as a BHV-4 that belongs to the American group. Additionally, the results of this study may indicate that the nervous system is one of the sites of viral latency in natural infection.
At a farm in Chiba Prefecture, in October 1996, an 18-month-old black Japanese heifer introduced from Hokkaido frothed abundantly at the mouth and showed nervous symptoms. Pathological investigation revealed perivascular cuffing, actively proliferating glia cells, and neuronophagias in the cerebrum.The lesions were present but relatively mild in the spinal cord. Immunoperoxidase staining showed the specific Japanese encephalitis virus (JEV) antigen in degenerated nerve cells. In addition, pathological findings indicated that the lungs were affected by fibrous pleuropneumonia.JEV was isolated from the brain and Pasteurella haemolytica from the lung. Two of four heifers reared in the same shed showed a significant rise in the hemagglutination-inhibiting antibody titer to JEV.
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