This is a simple, economic, sensitive stability indicating RP-HPLC method for the simultaneous estimation of Salmeterol Xinafoate and Fluticasone Propionate in bulk and pharmaceutical Formulation. The method was carried out on Octa-decyl C 18 column (5 μm, 25 cm x 4.6 mm, i.d) using methanol: water in the ratio of 70:30 and pH of the mobile phase up to 3 was adjusted with OPA at a flow rate of 0.8 ml/min. The wavelength for Salmeterol Xinafoate and Fluticasone Propionate at 232 nm was found to be appropriate. The linearity range was obtained in the concentration range of 20 to 100 μg/ml for Salmeterol Xinafoate (SLM) and 20 to 100 μg/ml for Fluticasone (FLT) respectively. The retention time of Salmeterol Xinafoate and Fluticasone Propionate were found to be 3.59 and 6.3 min, respectively. The regression equation for SLM and FLT were found to be as y = 0.009x-0.003 and y = 0.009x-0.031 with correlation coefficient (R 2) 0.999 and 0.999, respectively. The developed method is found to be robust, accurate and sensitive which can be used for estimation of combination of Salmeterol Xinafoate and Fluticasone Propionate in pharmaceutical dosage forms. The method was applicable for the quality control of the mentioned drugs in raw material, bulk drug and pharmaceutical formulations.
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