Development of RP-HPLC method for separation of atorvastatin calcium, amlodipine besylate and azilsartan medoxomil and its application to analyze their tablet dosage forms

., K.R. Wagh; Sonawane, Sandeep; Chhaajed, Santosh; Kshirsagar, Sanjay J.

doi:10.5958/2231-5691.2017.00023.5

Cited by 6 publications

(1 citation statement)

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“…An extensive literature review reveals that different analytical methods like HPTLC [4,5], RP-HPLC [6][7][8][9][10][11][12], RP-UPLC [13], UV [14], and LC-MS [15,16] have been reported for the estimation of AZL. HPTLC [17][18][19], UV [20][21][22] and RP-HPLC [23][24][25][26][27][28] methods have been reported for analysis of CLN in the bulk and pharmaceutical dosage form.…”

Section: Introductionmentioning

confidence: 99%

Liquid chromatographic method for simultaneous estimation of anti‐hypertensive drug combination Azilsartan Medoxomil and Cilnidipine

Solanki¹,

Panday²,

Shah³

et al. 2023

Separation Science Plus

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A combination of Azilsartan Medoxomil and Cilnidipine is prescribed in the treatment of Hypertension. An accurate and precise high‐performance liquid chromatographic method has been developed for the estimation of Azilsartan Medoxomil and Cilnidipine in a combined tablet dosage form. Symmetry C18 (250 × 4.6 mm, 5 μm) column was used as the stationary phase, and methanol:water (85:15 v/v; pH 3.5) was used as the mobile phase. The method was linear in the concentration range of 4–20 μg/ml for Azilsartan Medoxomil and 1–5 μg/ml for Cilnidipine with a correlation coefficient (r2) 0.995 for Azilsartan Medoxomil and 0.995 for Cilnidipine. The proposed method was validated with respect to linearity, accuracy, precision, and robustness as per International Council on Harmonization Q2 (R1) guideline. A forced degradation study was performed to find out the intrinsic stability of the molecules. The degradation products were well resolved from the drug peak and did not interfere with the analysis. The method was successfully applied for the analysis of Azilsartan Medoxomil and Cilnidipine in combined tablet formulation.

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Section: Introductionmentioning

confidence: 99%

Liquid chromatographic method for simultaneous estimation of anti‐hypertensive drug combination Azilsartan Medoxomil and Cilnidipine

Solanki¹,

Panday²,

Shah³

et al. 2023

Separation Science Plus

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HPTLC-Densitometric Estimation of Anti-hypertensive Drug Combination Azilsartan Medoxomil and Cilnidipine in Combined Dosage Form

Solanki¹,

Solanki²,

Desai³

et al. 2023

Analytical Chemistry Letters

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Stability Indicating HPTLC Method for Simultaneous Determination of Amlodipine Besylate and Lisinopril in Combined Dose Tablet Formulation

Wankhede¹,

Khobragade

Lote

et al. 2021

RJPT

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A combined dose tablet formulation containing Amlodipine besylate and Lisinopril is used for the treatment of essential hypertension. The present study reports development and validation of stability indicating high performance thin layer chromatographic method for simultaneous estimation of these drugs in combined dose tablet formulation. The two drugs were satisfactorily resolved on aluminum plates precoated with silica gel 60F254 using n-butanol : methanol: ammonia (4:4:1 v/v/v) as mobile phase. The Rf value for lisinopril and amlodipine besylate were 0.27±0.02 and 0.62±0.02, respectively. Densitometric evaluation of the separated bands was performed at 215nm. The calibration curves for lisinopril and amlodipine besylate were found to be linear in the concentration range of 1000-6000ng/band. The method was validated as per ICH guidelines for accuracy, precision, robustness, specificity, limit of detection and limit of quantitation. Statistical analysis proves that the method is suitable for simultaneous analysis of Lisinopril and Amlodipine besylate in pharmaceutical formulation without any interference from the excipients/degradant. The developed method offers several advantages such as sensitive, rapid, cost effective and less time consuming as compared to the reported methods. As the method could effectively separate the drugs from its degradation products, it can be employed as a stability indicating method.

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Development of RP-HPLC method for separation of atorvastatin calcium, amlodipine besylate and azilsartan medoxomil and its application to analyze their tablet dosage forms

Cited by 6 publications

References 0 publications

Liquid chromatographic method for simultaneous estimation of anti‐hypertensive drug combination Azilsartan Medoxomil and Cilnidipine

Liquid chromatographic method for simultaneous estimation of anti‐hypertensive drug combination Azilsartan Medoxomil and Cilnidipine

HPTLC-Densitometric Estimation of Anti-hypertensive Drug Combination Azilsartan Medoxomil and Cilnidipine in Combined Dosage Form

Stability Indicating HPTLC Method for Simultaneous Determination of Amlodipine Besylate and Lisinopril in Combined Dose Tablet Formulation

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