The present study was carried out to determine the prevalence of bovine sarcocystosis and identify the different species of cysts in cattle in different regions of Chittoor district, Andhra Pradesh. A total of 150 slaughtered cattle over a period of 1 year were examined both macroscopically and microscopically for the presence of infection. Out of 150 slaughtered cattle examined, macroscopic sarcocysts were observed in nine cattle and were exclusively found in oesophagus. Examination of tissue samples collected from different predilection sites from each of 150 cattle by pepsin-HCl digestion method revealed presence of live banana shaped bradyzoites under light microscopy in 134 cattle. Six cattle that were positive by macroscopic examination were also positive by microscopic examination of tissues. The overall prevalence of infection in cattle of Chittoor district was 91.33%. The prevalence of macroscopic and microscopic sarcocysts was 6.57 and 93.43% respectively. Statistically a significant relationship between the prevalence of infection among different age groups ( < 0.001) and no significant relationship ( > 0.001) between the prevalence of infection in male (91.76%) and female (90.76%) cattle was observed. In both male and females the prevalence of infection increased with age. Microscopically sections of tissues from cattle that were positive by tissue digestion technique revealed thin walled cysts (4.5 ± 0.5 µm) in 131 samples and thick walled cysts (0.5 ± 0.12 µm) in six cattle.
In the present study, the species specific determination of Sarcocystis cysts isolated from 137 naturally infected and slaughtered cattle from different regions of Chittoor district, Andhra Pradesh, India was achieved by PCR-RFLP. Diagnosis of sarcocysts in cattle was based on macroscopic examination and pepsin digestion method. Genomic DNA was extracted individually from bradyzoites of all 137 sarcocystosis positive cattle and all the isolates were characterized at 18S rRNA. Digestion of PCR amplicons (900 bp) with restriction endonuclease revealed 3 different electromorphs which were referred to Sarcocystis cruzi (513 and 343 bp), S. hirsuta (525, 241 and 141 bp) and S. fusiformis (532 and 335 bp). Sarcocystis cruzi (93.43%) was significantly more prevalent in comparison with the S. hirsuta (4.38%) and S. fusiformis (2.19%). Infection of cattle with S. hominis was not observed in the study area. Occurrence of S. fusiformis in cattle supports that Sarcocystis species of buffaloes are not strictly intermediate host specific however can infect cattle.
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