Stress responsive transcriptional regulation is an adaptive strategy of plants that alleviates the adverse effects of environmental stresses. The ectopic overexpression of Dehydration-Responsive Element Binding transcription factors (DREBs) either in homologous or in heterologous plants improved stress tolerance indicating the DRE/DREB regulon is conserved across plants. We developed 30 transgenic T(0) rice plants overexpressing OsDREB2A which were devoid of any growth penalty or phenotypic abnormalities during stressed or non-stressed conditions. Integration of T-DNA in the rice genome and stress inducible overexpression of OsDREB2A had occurred in these transgenic lines. Functional analyses of T(1)-3 and T(1)-10 lines revealed significant tolerance to osmotic, salt and dehydration stresses during simulated stress conditions with enhanced growth performance as compared to wild type. OsDREB2A, thus, confers stress tolerance in homologous rice system that failed in the heterologous Arabidopsis system earlier.
Facile approach to synthesize the metal oxide nanoparticles is getting an increased attention in various biomedical applications such as, to treat antibiotic resistant diseases. Magnesium oxide nanoparticles (MgOÁNPs) were synthesized by using Clitoria ternatea as the stabilizer in a green synthesis approach. The preliminary screening of MgOÁNPs in the presence of C. ternatea extract was observed by UV-visible spectrophotometer. X-ray diffraction (XRD) pattern have proved the crystalline nature of the MgOÁNPs; Photoluminescence (PL) measurement studies are used to identify the quality and defects in the crystal structure. FE-SEM with EDS has showed the size of 50-400 nm with specific binding energies. FT-IR has revealed the functional groups present in the plant extract and the peak at 521 cm -1 indicated the characteristic absorption bands of MgOÁNPs. The DPPH activity and reducing power assay of biologically synthesized MgOÁNPs could reach 65 % at a concentration of 150 lg/ml, respectively. From the results it was concluded that the biologically synthesized MgOÁNPs exhibit good antioxidant activity.
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