PCR based detection of (Sacc. & Syd.) -The causative agent of leaf blight and fruit rot disease of Brinjal ( L.)
Phomopsis vexans
Solanum melongenaI n t e r n a t i o n a l J o u r n a l o f L i f e S c i e n c e s 7 ( 1 ) : 2 0 1 3 ; 1 7 -2 0
Solanum melongena Phomopsis vexans
Phomopsis vexans
Phomopsis
growth ehamber at 25°C (day) and 20°C (night) with a 14-h photoperiod. The isolates were eultured on PDA at 25°C for 7 days. Stems were wounded at 10 em height with a drill. Eaeh isolate was inoeulated to 25 replieates per eultivar by plaeing a myeelia agar plug (4 to 5 mm diameter) in the hole and wrapping the stem with Parafilm. Plants treated identieally with sterile agar plugs were used as eontrols. Plants were then maintained at 100% relative humidity for 2 h. Both isolates indueed diseases symptoms and death of seedlings of both cultivars at a mean time of 37.5 days after inoeulation. No signifieant differenees between isolates or between cultivars were deteeted. Twenty eontrol plants similarly treated with sterile PDA dises did not display symptoms. C. parasítica was re-isolated from lesions, eonfirming Koeh's postulates. Andalusia has 14,000 ha of chestnut crops with high eommereial value due to their preeoeity. Dispersion of ehestnut blight in this zone ean reduee crop produetivity. To our knowledge, this is the first report of C. parasítica eausing chestnut blight in Andalusia (southern Spain), one of the few areas left in southwestern Europe free of ehestnut blight. . Plant Dis. 98:284, 2014; published online as http://dx.
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