PCR based detection of Phomopsis vexans (Sacc. &amp; Syd.) - The causative agent of leaf blight and fruit rot disease of Brinjal (Solanum melongena L.)

Jayaramaiah, K. M.; Mahadevakumar, S.; Raj, AP Charith; Janardhana, G. R.

doi:10.3126/ijls.v7i1.8173

Cited by 13 publications

(7 citation statements)

References 5 publications

(8 reference statements)

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“…Under normal weather conditions, fruit rot caused by Phomopsis vexans lead 12 to 25 per cent yield loss due to flower drop and fruit rot (Kannan et al, 1998). It reduces yield and marketable value of the crop from 20 to 50 per cent (Panwar et al, 1970;Jain and Bhatnagar, 1980;Kaur et al 1985;Das, 1998;Khan, 1999;Thippeswamy et al, 2005;Akhtar et al, 2008;Beura et al, 2008;Pandey, 2010;Jayaramaiah et al, 2013). Such a huge loss in fruit yield due to Phomopsis fruit rot was attributed to decreased fruit number (34.8 %) and fruit weight (17.0 %) as reported by Kidasha (2010) and due to poor seed germination and plant stand due to Phomopsis infection (Thippeswamy et al, 2005).…”

Section: Yield Losses Due To Phomopsis Vexansmentioning

confidence: 99%

“…The damping off, Phomopsis blight and fruit rot are caused by the Phomopsis vexans (Udayanga et al, 2011;Jayaramaiah et al, 2013) and was first reported from Gujarat in 1914 and then from almost all the states wherever brinjal is grown (Rangaswamy and Mahadevan, 2002). Phomopsis vexans primarily infect the seed coat (2%), cotyledons (4 %) and embryo axis (4 %) and attacks all the above ground plant parts (Thippeswamy et al, 2005).…”

mentioning

confidence: 99%

“…In Karnataka, Mahadevakumar and Janardhan (2015) reported a wide range of Phomopsis blight from 7.7 to 30.5 per cent with fruit rot ranging from 21.0 to 64.6 per cent. Jayaramaiah et al, (2013) reported that Phomopsis vexans cause leaf blight to the extent of 5 to 23 per cent and fruit rot to the extent of 30 to 60 per cent. Recently Mahadevakumar et al, (2017) reported leaf blight incidence (8 to 25 %) and fruit rot incidence (15 to 62 %) caused by P. vexans.…”

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confidence: 99%

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Management of Fruit Rot of Brinjal Caused by Phomopsis vexans

Reddy¹,

Jakhar²,

Dahiya³

2018

Int.J.Curr.Microbiol.App.Sci

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Brinjal (Solanum melongena L.) belongs to family Solanaceae. It is the most important vegetable in India (Sekara et al., 2007) and described as "king of vegetables" due to its wide usage in Indian foods (Choudhary and Gaur, 2009;Singh et al., 2014). Area, production and productivity of brinjal have increased over the years, although increment in productivity remained relatively less over the years (Anon, 2015 and NHB, 2017). Globally, India stands 2 nd in brinjal production; and in India, brinjal constitutes 8.1 per cent of vegetable production (Anon, 2012; Anon, 2013; Anon, 2015). Presently, brinjal ranks 4 th important vegetable crop in India after potato, onion and tomato. Brinjal is cultivated in an area of 0.72 million ha with a production of 12.32 million tones and productivity of 16.95 t ha -1 during the year 2016-17 (NHB, 2017). Although, the productivity of brinjal in India has increased, it is far less than the productivity in a few other countries viz., China, Japan, Turkey, Egypt and Italy (Anon, 2012). Such lower production could be attributed to several environmental, edaphic and plant factors. Among the various production constraints in brinjal, diseases are the prominent factor in

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Section: Yield Losses Due To Phomopsis Vexansmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Management of Fruit Rot of Brinjal Caused by Phomopsis vexans

Reddy¹,

Jakhar²,

Dahiya³

2018

Int.J.Curr.Microbiol.App.Sci

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“…It is widely planted in China, India, Pakistan, Menggara, the Philippines and other countries ( Kumar et al., 2011 ). Eggplant brown streak (Phomopsis blight of eggplant) caused by P. vexans is an important disease that limits eggplant production ( Rangaswami and Madevan, 1998 ; Khan, 1999 ; Akhtar and Chaube, 2006 ; Jayaramaiah et al., 2013 ), causing leaf and stem death or fruit rot. The disease was shown to decrease the production of eggplant by 20-50% in one season ( Akhtar and Chaube, 2006 ; Pandey, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Discovery and exploration of widespread infection of mycoviruses in Phomopsis vexans, the causal agent of phomopsis blight of eggplant in China

et al. 2022

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Phomopsis vexans, which causes Phomopsis blight of eggplant, has been reported worldwide. To study the biocontrol of this disease, 162 leaf and fruit samples of eggplant Phomopsis blight were collected from Hunan, Hubei, Jiangxi, Sichuan, Zhejiang, Fujian, Guangdong and Anhui Provinces from 2017 to 2019. Eighty-seven pathogenic fungus isolates were identified as P. vexans. The following studies were conducted: screening of sporulation medium, spore morphology analysis, mycovirus detection and identification of novel mycoviruses in these isolates. The results showed that eggplant tissue medium was the most suitable medium for rapid sporulation, and all isolates had mycoviruses consisting of mainly mixed infections. The genome of these mycoviruses varied from 1-15 kb. Five novel mycoviruses infecting P. vexans were obtained, including “Phomopsis vexans fusarivirus 1” (PvFV1), “Phomopsis vexans ourmia-like virus 1” (PvOLV1), “Phomopsis vexans endornavirus 2” (PvEV2), “Phomopsis vexans partitivirus 1” (PvPV1) and “Phomopsis vexans victorivirus L1” (PvVVL1). Thus, PvVVL1 displays a unique genome structure, and this is the first report of a victorivirus consisting of two segments and of a deltapartitivirus infecting the fungus host.

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“…Phomopsis vexans primarily infect seed coat (2%), cotyledons (4%), embryo axis (4%) and attacks all the above‐ground plant parts (Thippeswamy et al ). Losses caused by P. vexans is mainly attributed to decreased fruit number, fruit weight, flower drop and fruit rot which reduces yield and marketable value of crop by 20–70% (Cristina ; Jayaramaiah et al ).…”

Section: Introductionmentioning

confidence: 99%

Specific PCR‐based detection of Phomopsis vexans the cause of leaf blight and fruit rot pathogen of Solanum melongena L.

Udayashankar

Nayaka

Archana

et al. 2019

Lett Appl Microbiol

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Significance and Impact of the Study: Phomopsis vexans is an important seed-borne pathogenic fungus responsible for leaf blight and fruit rot in brinjal. Current detection methods, based on culture and morphological identification is time consuming, laborious and are not always reliable. A PCR-based diagnostic method was developed with species-specific primers designed based on sequence data of a region consisting of the 5Á8S RNA gene and internal transcribed spacers, ITS 1 and ITS 2 of nuclear ribosomal RNA gene (rDNA) repeats of P. vexans. AbstractLeaf blight and fruit rot disease caused by Phomopsis vexans is a devastating disease of brinjal. The detection of P. vexans in plant parts and seeds of brinjal can be complicated, mainly when the inoculum is present at low levels and/or overgrown by fast-growing saprophytic fungi or other seed-borne fungi. A PCR-based diagnostic method was developed with specific primers designed based on sequence data of a region consisting of the 5Á8S RNA gene and internal transcribed spacers, ITS 1 and ITS 2 of nuclear ribosomal RNA gene (rDNA) repeats of P. vexans. The efficiency and specificity of primer pairs PvexF/PvexR designed were established by PCR analysis of DNA from P. vexans strains isolated from India and fungal isolates of other genera. A single amplification product of 323-bp was detected from DNA of P. vexans isolates. No cross-reaction was observed with any of the other isolates tested. The specific primers designed and employed in PCR detected P. vexans up to 10 pg from DNA isolated from pure culture. This is the first report on the development of species-specific PCR assay for identification and detection of P. vexans. Thus, PCR-based assay developed is very specific, rapid, confirmatory and sensitive tool for the detection of pathogen P. vexans at early stages.

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PCR based detection of Phomopsis vexans (Sacc. & Syd.) - The causative agent of leaf blight and fruit rot disease of Brinjal (Solanum melongena L.)

Cited by 13 publications

References 5 publications

Management of Fruit Rot of Brinjal Caused by Phomopsis vexans

Management of Fruit Rot of Brinjal Caused by Phomopsis vexans

Discovery and exploration of widespread infection of mycoviruses in Phomopsis vexans, the causal agent of phomopsis blight of eggplant in China

Specific PCR‐based detection of Phomopsis vexans the cause of leaf blight and fruit rot pathogen of Solanum melongena L.

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