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Biodegradation is a process in which microorganisms are committed to transform toxic chemicals into less toxic or harmless. Three bacterial strains, Escherichia coli (E. coli), Micrococcus sp., and Clostridium sp. were isolated from contaminated soil found near the automobile service station and tested for their ability to degrade the waste oil sludge found near automobile service station. The results collectively indicating that these bacterial strains have considerable potential for bioremediation of waste oil sludge. The present study also demonstrates that, among the three organisms, E. coli showed the best oil degrader, when compared to other two bacterial species.

The new Schiff base ligand (Z)-2-(methylthio)-N-(pyridin-2-ylmethylene)aniline MPMA derived from pyridine-2- carboxaldehyde and 2- (methylthio) aniline and its Cu(II), Co(II) and Zn(II) metal complexes were prepared and characterized by spectral, magnetic and electrochemical studies. The spectral studies revealed that the ligand MPMA was tridentate and coordinated to the metal through azomethine nitrogen atom, pyridine nitrogen atom and sulphur atom from amine, forming octahedral geometry for Cu2+,Co2+ and tetrahedral geometry for Zn2+ ions. It was further supported by molar conductance measurement, which indicated that the above-mentioned complexes 1 and 2 were formed in 1:2 metal- ligand ratio and complex 3 was formed in 1:1 metal-ligand ratio. The electrochemical study explored that the metal ions undergo quasi reversible redox reactions by two electron transfer processes. The bioefficacy of the Schiff base and their metal complexes were studied in-vitro against the growth of microbes to assess their antimicrobial potential. The antioxidant activity of the ligand and the metal complexes have also been studied. The antidiabetic activity of the ligand and metal complexes were screened against α-amylase enzyme and αglucosidase enzyme and compared with standard drug acarbose.

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