DNA binding and DNA cleavage characteristics of synthesized mixed ligand copper(II) complexes of the type [Cu(HL)2Cl2](1), [Cu(HL)(bipy)Cl2](2), [Cu(HL) (phen)Cl2](3), [Cu(HL)2Br2](4), [Cu(HL) (bipy)Br2](5), [Cu(HL)(phen)Br2](6) (Where, L= 2- (methylsulfanyl)-N-(1H-pyrrole-2-ylmethylidene) aniline, bipy= 2,2’-Bipyridine, Phen= 1,10- Phenanthroline) have been investigated in detail. DNA binding properties of copper(II) complexes have been investigated by electronic absorption spectroscopy. Results suggest that the copper (II) complexes were bound to DNA by an intercalation binding mode. The in vitro antimicrobial activities of the synthesised compounds have been tested against the bacteria Escherichia Coli and the fungi Aspergillus niger by using disc agar diffusion method. The minimum inhibitory concentration (MIC) value against the growth of the above microorganism is much larger for mixed ligand copper(II) complexes. The cleavage activities of complexes (1), (2), (3), (4), (5) and (6) were carried out on double-stranded pUC19 DNA using gel electrophoresis. The complexes show better nuclease activity for mixed ligand copper(II) complexes.
The new Schiff base ligand (Z)-2-(methylthio)-N-(pyridin-2-ylmethylene)aniline MPMA derived from pyridine-2- carboxaldehyde and 2- (methylthio) aniline and its Cu(II), Co(II) and Zn(II) metal complexes were prepared and characterized by spectral, magnetic and electrochemical studies. The spectral studies revealed that the ligand MPMA was tridentate and coordinated to the metal through azomethine nitrogen atom, pyridine nitrogen atom and sulphur atom from amine, forming octahedral geometry for Cu2+,Co2+ and tetrahedral geometry for Zn2+ ions. It was further supported by molar conductance measurement, which indicated that the above-mentioned complexes 1 and 2 were formed in 1:2 metal- ligand ratio and complex 3 was formed in 1:1 metal-ligand ratio. The electrochemical study explored that the metal ions undergo quasi reversible redox reactions by two electron transfer processes. The bioefficacy of the Schiff base and their metal complexes were studied in-vitro against the growth of microbes to assess their antimicrobial potential. The antioxidant activity of the ligand and the metal complexes have also been studied. The antidiabetic activity of the ligand and metal complexes were screened against α-amylase enzyme and αglucosidase enzyme and compared with standard drug acarbose.
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