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The green synthesis of NPs through plant extracts can be a modest, one-pot alternative synthesis to the conventional physical or chemical method. The prime focus of this study is to produce MNPs by the reducing effect of Bauhinia tomentosa leaf extract, and it was immobilized in porcine pancreatic lipase (PPL). Synthesized NPs were characterized by field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) and Raman spectroscopy, UV–Vis Spectrometry, Thermogravimetry, and Differential Scanning Calorimeter (DSC), Zeta potential test, VSM, BET and Fourier Transform Infrared Spectroscopy (FTIR). The effect of process parameters was studied, about the efficiency of immobilization are enzyme stability, the extent of enzyme reusability, its separation from products, the activity of immobilized enzyme, recovery, and its loss. Finally, the immobilized lipase was used for the synthesis of 1,3-diolein using enzyme-mediated esterification of oleic acid and glycerol. Under optimized condition (reaction temp-55 $$^\circ $$ ∘ C; molar ratio-2.5:1; pH-7) diolein yield was achieved to be 94%. Therefore, this work was further used for the industrial production of 1,3-diacylglycerol since a perfect enzyme-catalyzed process was observed.

This paper presents an empirical analysis on the use of aqueous extract of Centratherum punctatum Cass (Asteraceae) for the production of silver nanoparticles (AgNPs) from aqueous silver nitrate. Phytochemical analysis of the extract revealed the presence of essential components such as flavonoids, alkaloids, carbohydrates, tannins and vitamin C, some of which serve as efficient reducing and capping agents for the reduction of silver nitrate to silver nanoparticles. The AgNPs synthesized were characterized by UV -Vis spectroscopy, FT-IR, XPS, SEM, TEM, Zetasizer and TG-DSC analyzer. In the present study, attempts have also been made to investigate the anti-inflammatory and anti-oxidant activity of the synthesized AgNPs. Anti-oxidant activity was assessed using DPPH method. Anti-inflammatory potential was evaluated through in vitro inhibition of protein denaturation, protease activity and improved membrane stabilization property.

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