A method was developed and a collaborative study was performed under the auspices of the International Commission of Uniform Methods for Sugar Analysis. The collaborators used high-performance anion-exchange chromatography (HPAEC) to determine trace amounts of glucose and fructose in 3 raw and 3 refined sugar samples provided as blind duplicates. Fourteen laboratories participated in the study. Although difficulties were experienced by a few analysts, 10 laboratories reported positive results. Average repeatabilities and reproducibilities for glucose and fructose in raw sugar were slightly above 5 and 10%, respectively, and the average Horwitz ratios were well under 2. Average repeatabilities and reproducibilities for glucose and fructose in refined sugar were 10 and 22%, respectively; although the Horwitz ratios were >2, they were marginal (2.8). The HPAEC results agreed with results obtained by gas chromatography in an independent laboratory. Because the method was collaboratively studied according to the protocol of the International Union of Pure and Applied Chemistry for collaborative studies, and the results meet its criteria, it is recommended that the method be adopted Official First Action by AOAC INTERNATIONAL.
A collaborative study of an ion chromatographic tech’ nique of high-performance anion-exchange with pulsed amperometric detection has been completed. The procedure was used to determine sucrose, glucose, and fructose in 8 cane molasses samples and sucrose in 5 beet molasses samples. Eleven laboratories took part in the study. Repeatability and reproducibility values for sucrose, glucose, and fructose were excellent for both types of samples. All criteria for accepting the method for sugars in both beet and cane molasses were met. The ion chromatographic method for determination of sugars in beet and cane final molasses has been adopted first action by AOAC INTERNATIONAL.
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