Quantitative gas chromatographic analysis of sucrose in the presence of sugar oximes using a buffered oximation reagent and glass capillary columns

Schäffler, K. J.; Boil, P. G. M. du

doi:10.1016/s0021-9673(00)89934-3

Cited by 12 publications

(2 citation statements)

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“…Reproducible results have been obtained in the quantitation of glucose, fructose, sucrose and maltose content of breakfast [32] and granola cereals [33]. The hydrolysis-stability study of sucrose, (under a two step derivatization process of other sugars present), [34] was also followed by the elution of the TMS ether/oxime derivatives. In particular good results were achieved in the identification and quantitation of the raffinose family of oligosaccharides (sugars of DP 1-DP 5) in soy samples [35,36].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantitation of mono-, di-and trisaccharides as their TMS ether oxime derivatives by GC-MS: I. In model solutions

Molnár-Perl

Horváth

1997

Chromatographia

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SummaryThis paper describes the separation and quantitation of mono-di-and trisaccharides in model solutions. Optimized conditions for identification and determination of common sugar constituents in several natural matrices by GC-MS, from one solution by one injection, are given. Applying a 52 min temperature program to a 30 m capillary column and taking advantage of the stable ratios ofthe syn-and anti oximes eluting either alone or together with one or two other anomers: provides the basis of calculation for co-eluting compounds. Saccharides (5-200 ng), such as fructose, glucose, sucrose, trehalose, cellobiose, maltose, turanose, gentiobiose, palatinose, melibiose, isomaltose, erlose, raffinose, melezitose, maltotriose, panose and isomaltotriose, in the presence of each other in various ratios, have been measured as silyloxime compounds with a reproducibility of < 5 R.S.D. %. The possibility of measuring all these saccharides as their TMS methoximes is also presented.

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Section: Introductionmentioning

confidence: 99%

Simultaneous quantitation of mono-, di-and trisaccharides as their TMS ether oxime derivatives by GC-MS: I. In model solutions

Molnár-Perl

Horváth

1997

Chromatographia

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“…However, owing to polarity, hydrophilicity and low volatility, carbohydrates have to be converted into volatile and stable derivatives, i.e. acetate or trimethylsilane (TMS) derivatives (Adam and Jennings, 1975; Bartolozzi et al ., 1997; Guignard et al ., 2005; Li and Schuhmann 1980; Low and Sporns, 1988; Molnár‐Perl and Horváth, 1997; Molnár‐Perl and Szakács‐Pinter, 1985; Molnár‐Perl et al ., 1984, 1990; Morita and Montgomery, 1978; Rojas‐Escudero et al ., 2004; Schäffler and Du Boil, 1981; Silva and Ferraz, 2004; Toba and Adachi, 1977) before GC analysis. Generally, silylation is the most common and versatile method for the derivatisation of organic compounds containing active hydrogen atoms and resulting in products with reduced polarity (fewer dipole–dipole interactions), enhanced volatility and increased thermal and catalytic stability necessary for optimal sensitivity and resolution (Halket and Zaikin, 2003).…”

Section: Introductionmentioning

confidence: 99%

Determination of carbohydrates in medicinal plants‐comparison between TLC, mf‐MELDI‐MS and GC‐MS

Qureshi

Stecher

Sultana

et al. 2011

Phytochemical Analysis

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Monosaccharides and Sugar Alcohol Analysis

Sturgeon

2000

Encyclopedia of Analytical Chemistry

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Carbohydrates, including monosaccharides and sugar alcohols, are widely distributed in nature, being found in plants, animals and micro‐organisms. They occur free, or in glycosidic linkage as polysaccharides or glycoconjugates (glycoproteins and glycolipids). It is important that suitable and sensitive methods are available for the analysis of these sugars, as in many cases only very small (microgram) amounts of material may be available. Polysaccharides and glycoconjugates must first be depolymerized to produce the monosaccharides quantitatively. The total carbohydrate released in these reactions may be determined by spectrophotometric or enzymic procedures. Numerous separation and detection methods are available to measure the individual sugar components. Paper and thin‐layer chromatography were two of the earliest techniques available, giving mainly qualitative values for individual sugar components. Major advances were made first in gas–liquid chromatography (GLC) and then with various types of liquid chromatography (LC) in the separation of individual sugars. High‐performance liquid chromatography (HPLC) and capillary electrophoresis (CE) are now two of the most commonly used techniques in sugar chromatography. Highly sophisticated detection systems, such as mass spectrometry (MS) for gas–liquid chromatographic separations, and spectrophotometric, fluorometric and electrochemical detection systems for LC of carbohydrates with suitable chromophores or fluorophores have been developed. Carbohydrates have been derivatized for use either in precolumn or postcolumn chromatography, resulting in the detection limit in the most sensitive systems approaching yoctomole levels for individual sugars.

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Quantitative gas chromatographic analysis of sucrose in the presence of sugar oximes using a buffered oximation reagent and glass capillary columns

Cited by 12 publications

References 3 publications

Simultaneous quantitation of mono-, di-and trisaccharides as their TMS ether oxime derivatives by GC-MS: I. In model solutions

Simultaneous quantitation of mono-, di-and trisaccharides as their TMS ether oxime derivatives by GC-MS: I. In model solutions

Determination of carbohydrates in medicinal plants‐comparison between TLC, mf‐MELDI‐MS and GC‐MS

Monosaccharides and Sugar Alcohol Analysis

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