The PEL1/PGS1 gene of the yeast Saccharomyces cerevisiae is essential for the viability of rho-/rho degrees mutants and the normal cardiolipin content of cells. The PEL1-GFP fusion gene has been found to complement the pel1/pgs1 mutation and its fluorescent protein was localized to mitochondria similarly to the beta-galactosidase activity of a protein encoded by the PEL1-lacZ fusion gene. The expression of the PEL1-lacZ reporter gene was repressed in cells grown in the presence of inositol and choline, reduced in the ino2 and ino4 strains, but constitutive in the opi1 null-mutant strain. The results demonstrate that Pel1p, playing a vital role in cells impaired in the mitochondrial DNA, is localized in the mitochondria and expressed in response to inositol and choline.
By ethyl methanesulphonate mutagenesis of the yeast Kluyveromyces lactis we have isolated five nuclear mutants that were unable to grow on non-fermentable carbon sources. The mutations were found to belong to three complementation groups. After functional complementation of the mutation in one of these mutants we have cloned the structural gene for cytochrome c1, named KlCYT1. This gene has been assigned to chromosome VI and its nucleotide sequence exhibited 74.3% identity to the homologous gene of S. cerevisiae.
Thiadiazole derivatives R 0300Improved Synthesis, Antibacterial Activity and Potential Carcinogenicity of 5-Amino-1,2,4-thiadiazol-3(2H)-one. -Improved procedures for the synthesis of thiobiuret (II), some of its methyl derivatives [cf. (VI), (X), (XII)] and the title thiadiazole (III) are described. Moreover, studies on the reductive cleavage, the alkaline degradation course and the antibacterial activity of thiadiazole (III) as well as the potential carcinogenicity of all the compounds prepared are given. -(PISKALA, A.; VACHALKOVA, A.; MASOJIDKOVA, M.; HORVATHOVA, K.; OVESNA, Z.; PACES, V.; NOVOTNY*, L.; Pharmazie 59 (2004) 10, 756-762; Fac. Pharm., Kuwait Univ., 13110 Safat, Kuwait; Eng.) -M. Bohle 04-136
The aim of the synthesis of substituted furo[3,2-b]- and furo[2,3-b]pyrroles was to discover biologically active compounds. On that account, their potential carcinogenicity was tested by DC polarographic assay in the presence of α-lipoic acid. Only one of the studied compounds showed a marginal carcinogenic activity, the other exhibited only a negligible carcinogenic potential under the conditions studied. Their apparent ionization constants, determined by spectrometric titration, were correlated with their structure. The results confirm that the presence of methyl, furyl and/or phenyl groups affects the ionization.
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