A new external ion-beam system was developed and combined with a light ion-microbeam system in JAERI Takasaki. The system is designed for micro-particle-induced X-ray emission analysis of biological samples in air environment with 1 micron spatial resolution. One of the most serious problems in keeping such a high spatial resolution is multiple scattering in a beam exit window. Thin Kapton film (7.5 microns thick) was adopted as the exit window as well as a sample backing foil to minimize the distance between the film and samples. The lifetime of the foil under ion irradiation and spatial resolution of the external microbeam were investigated. The results shows that the film can endure sufficient long-time irradiation to take elemental maps and the resolution can be kept nearly 1 micron.
Reduced activation ferritic-martensitic steel F82H was irradiated with 5 keV helium ions to introduce the surface damage. After the irradiation, the irradiated F82H was continuously exposed to 1.7 keV deuterium ions. The effects of the damages introduced by the helium ion irradiation on deuterium retention and desorption behavior of F82H were investigated. The HD desorption had a peak at around 500 K, which was shifted to the high temperature region when the helium ion fluence increased. The decrease in HDO desorption at the high fluence region was observed. This might be owing to the oxygen depletion on the surface by the sputtering. The amount of retained deuterium of the F82H gradually increased with the helium ion fluence and saturated at 7.2 dpa.
We developed a PIXE analysis system which provides spatial distribution images of elements in a region of several cm2 with a spatial resolution of < 0.5 mm. We call this system a submilli-PIXE camera. This system consists of a submilli-beam line, beam scanners and a data acquisition system in which the X-ray energy and the beam position are simultaneously measured. We demonstrate the usefulness of the submilli-PIXE camera by analyzing the surface of a shell and of granite.
Since the cellular metabolism has not yet been fully elucidated, it is very useful to get the information of elemental distributions in a single cell. We applied a micro-PIXE camera to obtain a picture presented with density of an element in a cell. Our micro-PIXE camera is on the basis of PIXE analysis using micro ion beams with the spot size of less than 1 u.m and the samples can be analyzed in air, so the size and the state of samples are not limited. Here, we analyzed the spatial distributions of elements in a single cell of animal for a medical application. Bovine aortic endothelial cells(BAECs) were cultured in culture medium containing bromodeoxyuridine(BrdU) which is known to be used in the DNA synthesis. We could take the elemental maps of phosphorus, potassium and sulfur which present well a shape of cell and a position of nucleus in this cell. Moreover, we obtained the picture of Br, that is, BrdU. It was found that BrdU was locally concentrated in two or three regions in the nucleus.
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