Escherichia coli strains are important causes of diarrheal disease in the world and remain a major public health problem of animals and human. Sixty seven samples from different kind of food, water, soil and composit were screened for the detection of verotoxin-producing E.coli (VTEC) in the Egyptian markets from different location. Result showed that none of the samples gives positive results for VTEC (O104) (vt1 and vt2) detection. All samples from the Egyptian markets are negative for Vero cyto toxin producing E. coli (O104) with percentage of 100.