Background: Pannexins functions in skeletal myogenesis are unknown.Results: Panx1 and Panx3 species are co-expressed in skeletal muscle. Their levels are modulated during myoblast differentiation regulating either myoblast proliferation and/or differentiation status.Conclusion: Panx1 and Panx3 channels are novel regulators of skeletal muscle myoblast differentiation and proliferation.Significance: Skeletal muscle development and health depend on functional pannexin channels.

Direct dynamics simulations are reported for quantum mechanical (QM)/molecular mechanical (MM) trajectories of N-protonated diglycine (gly(2)-H(+)) colliding with chemically modified perfluorinated octanethiolate self-assembled monolayer (SAM) surfaces. The RM1 semiempirical theory is used for the QM component of the trajectories. RM1 activation and reaction energies were compared with those determined from higher-level ab initio theories. Two chemical modifications are considered in which a head group (-COCl or -CHO) is substituted on the terminal carbon of a single chain of the SAM. These surfaces are designated as the COCl-SAM and CHO-SAM, respectively. Fragmentation, peptide reaction with the SAM, and covalent linkage of the peptide or its fragments with the SAM surface are observed. Peptide fragmentation via concerted CH(2)-CO bond breakage is the dominant pathway for both surfaces. HCl formation is the dominant species produced by reaction with the COCl-SAM, while for the CHO-SAM a concerted H-atom transfer from the CHO-SAM to the peptide combined with either a H-atom or radical transfer from the peptide to the surface to form singlet reaction products is the dominant pathway. A strong collision energy dependence is found for the probability of peptide fragmentation, its reactivity, and linkage with the SAM. Surface deposition, i.e., covalent linkage between the surface and the peptide, is compared to recent experimental observations of such bonding by Laskin and co-workers [Phys. Chem. Chem. Phys. 10, 1512 (2008)]. Qualitative differences in reactivity are seen between the COCl-SAM and CHO-SAM showing that chemical identity is important for surface reactivity. The probability of reactive surface deposition, which is most closely analogous to experimental observables, peaks at a value of around 20% for a collision energy of 50 eV.

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Laser Ar-Ar dating of lunar melts improves chronology.

New geologic sketch maps and digital elevation models for King crater and the surrounding lunar farside highlands were created from Lunar Reconnaissance Orbiter Camera (LROC) Wide‐angle Camera (WAC) and Narrow Angle Camera (NAC) images. NAC images reveal that high volume impact melt accumulations exhibit evidence of dynamic processes during and following emplacement that resulted in downwarped zones, and other morphologic anomalies visible at the 50 cm pixel scale. Among the most significant of these forms are negative relief features, some of which may represent evidence for near‐surface caverns, offering points of access to subsurface environments and possible shelter from surface hazards. Other negative relief features may represent regions of extension and separation in response to possible subsurface drainage, together with isostatic readjustments, contraction, and/or compaction, in the cooling impact melt. Crater counts on the continuous ejecta blanket suggest a Late Eratosthenian to Copernican age for King crater, which is older than the estimate of Young (1977), but consistent with those of others.