The main furanocoumarins from the roots of PEUCEDANUM PALUSTRE were separated with a RP-HPLC method. The mobile phase for the separation of the eight main compounds (oxypeucedanin hydrate, bergapten, oxypeucedanin, isobyakangelicin angelate, ostruthol, imperatorin, isoimperatorin, and columbianadin) was optimized with the "PRISMA" model, which is a multi-solvent optimization system. The chromatographic method can be applied for quantitative determination, as was demonstrated for different samples of P. PALUSTRE. The purity and identity of the peaks were controlled by diode-array detection and comparison with standards.
Isobyakangelicin angelate, a new furanocoumarin and the five known coumarins, columbianadin, (+)-oxypeucedanin, (±)-oxypeucedanin hydrate, ostruthol, and isoimperatorin, were isolated from roots of Peucedanum palustre (L.) Moench using medium-pressure liquid chromatography [MPLC] with normal phase and subsequently with reversed phase as stationary phases. The mobile phases were optimized in TLC and HPLC preassays according to the "PRISMA" system and then transferred without modification to preparative MPLC separations. The structures of the compounds were elucidated by a combination of spectroscopic methods, among others the rotating frame Overhauser enhancement spectroscopy [ROESY] experiment.
The main flavonoid glycosides from BETULAE folium are separated with a RP-HPLC method. The mobile phase for the separation of the seven most important compounds (rutin, hyperin, quercetin-3-arabinofuranoside, quercetin-3-arabinopyranoside, quercetin-3-rhamnoside, quercetin-3-glucuronide, and myricetin-3-galactoside) was optimized with the "PRISMA" model, which is a multisolvent optimization system. The isocratic separations were finished within 30 minutes and the peak purity was controlled with diode-array detection. The method can be applied for quantitative determination, as was demonstrated for different samples of B. PENDULA and B. PUBESCENS.
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