Rotachrom-Analytical Separations of Naturally Occuring Compounds

Nyiredy, Sz.; Dallenbach-Tölke, K.; Nyiredy-Mikita, K.; Mészáros, Szilvia; Sticher, O.

doi:10.1055/s-2007-969329

Cited by 5 publications

(6 citation statements)

References 1 publication

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“…To eliminate adsorbed air and/or gas from the layer in OPLC, a prerun was carried out for 5 mm, with a flow rate of 1 mI/mm (19). The MPLC column was dry filled (20) and equilibrated with n-hexane (700 ml) before the actual separation in order to transfer the mobile phase from analytical OPLC (21,22). 5 g of the residue was chromatographed over silica gel (TLC Kieselgel OF2, particle size 15 Ism, Merck, Darmstadt, FRG) using a MPLC column (36 x 460 mm), and the optimized mobile phase at a flow rate of 2.0 mI/mm.…”

Section: Mplc Isolation and Purification Of Compoundsmentioning

confidence: 99%

“…They were subjected to RP chromatography (Sepralyte C-18, particle size 25-40 tm, Analytichem, Harbor City, USA) using MPLC column (18.5 x 713 mm) with a flow rate of 3 mlfmin. The mobile phase was transferred from analytical HPLC to preparative MPLC (22) so that the selectivity remained the same in preparative separations whereas the ST was varied: for compound 1, 5T = 1.0; for compound 2, 5T = 1.3; for compound 3, ST = 1.43; for compound 4, 5T = 1.6, and for compound 5, ST = 1.8. The purification was finished in all cases within 15 hours.…”

Section: Mplc Isolation and Purification Of Compoundsmentioning

confidence: 99%

See 1 more Smart Citation

Isobyakangelicin Angelate; A Novel Furanocoumarin fromPeucedanum palustre

Vuorela¹,

Erdelmeier²,

Nyiredy³

et al. 1988

Planta Med

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Isobyakangelicin angelate, a new furanocoumarin and the five known coumarins, columbianadin, (+)-oxypeucedanin, (±)-oxypeucedanin hydrate, ostruthol, and isoimperatorin, were isolated from roots of Peucedanum palustre (L.) Moench using medium-pressure liquid chromatography [MPLC] with normal phase and subsequently with reversed phase as stationary phases. The mobile phases were optimized in TLC and HPLC preassays according to the "PRISMA" system and then transferred without modification to preparative MPLC separations. The structures of the compounds were elucidated by a combination of spectroscopic methods, among others the rotating frame Overhauser enhancement spectroscopy [ROESY] experiment.

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Section: Mplc Isolation and Purification Of Compoundsmentioning

confidence: 99%

Section: Mplc Isolation and Purification Of Compoundsmentioning

confidence: 99%

Isobyakangelicin Angelate; A Novel Furanocoumarin fromPeucedanum palustre

Vuorela¹,

Erdelmeier²,

Nyiredy³

et al. 1988

Planta Med

Self Cite

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show abstract

“…One of the earliest is rotational planar chromatography (RPC), in which the TLC plate is rotated at a high angular velocity to generate a centrifugal force that moves the mobile phase through the layer in a circular profile. The method is now well established, and apparatuses for preparative or analytical separations are commercially available (4,9). RPC shares many of the advantages of other planar chromatographic methods, including simultaneous separation of multiple samples and the removal of solvent before scanning the plate.…”

Section: What Is Planar Chromatography?mentioning

confidence: 99%

Peer Reviewed: Forced-Flow Techniques in Planar Chromatography

Nurok¹

2000

Anal. Chem.

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“…Window diagrams, simplex methods, statistical design, and the prisma method have emerged in the past few years as powerful solvent optimization strategies for TLC (18)(19)(20). These methodological guides and computational techniques minimize the number of trial-and-error experiments required to identify the most valuable solvent systems for a separation.…”

Section: Theorymentioning

confidence: 99%