HPLC-Analyse der Flavonoidglykoside ausBetulaefolium¹

Abstract

The main flavonoid glycosides from BETULAE folium are separated with a RP-HPLC method. The mobile phase for the separation of the seven most important compounds (rutin, hyperin, quercetin-3-arabinofuranoside, quercetin-3-arabinopyranoside, quercetin-3-rhamnoside, quercetin-3-glucuronide, and myricetin-3-galactoside) was optimized with the "PRISMA" model, which is a multisolvent optimization system. The isocratic separations were finished within 30 minutes and the peak purity was controlled with diode-array detection. The method can be applied for quantitative determination, as was demonstrated for different samples of B. PENDULA and B. PUBESCENS.

Effect of Sample Preparation Method on Birch (Betula pendula Roth) Leaf Phenolics

“…The identification of (+)-catechin, chlorogenic acid, and flavonoid glycosides was based on comparison of retention and spectral characteristics of the corresponding peaks with standard compounds. Myricetin 3-galactoside, quercetin 3-glucuronide, and hydroxycinnamic acids were tentatively identified by their UV spectra and relative retention order (Harborne and Boardley, 1984;Dallenbach-To ¨lke et al, 1987). A major peak (Z 1 ) with almost identical spectrum with picein (glucoside of 4-hydroxyacetophenone), but eluting later than picein, is probably 3,4′-dihydroxypropiophenone 3-β-D-glucopyranoside, which has been reported from leaves of Betula alba (Tschesche et al, 1974), B. platyphylla var.…”

Comparison of methods for the extraction of flavonoids from birch leaves (Betula pendula Roth.) carried out using high-performance liquid chromatography

“…The identification of (-l-)-catechin, chlorogenic acid, hyperin, quercetin 3-arabinopyranoside, and quercitrin was based on comparisons of the retention and the spectral characteristics of the corresponding peaks with those of the standards. Quercetin 3-glucuronide and quercetin 3-arabinofuranoside were tentatively identified by their characteristic UV-vis spectra and relative elution order (Har borne and Boardley, 1984;Dallenbach-Tolke et al, 1987). The spectra of peaks 4 and 5 were characteristic of a myricetin 3-glycoside.…”

Hawthorn (Crataegus): Biological Activity and New Strategies for Quality Control

“…Glasl and Becker (26) demon strated that the absorption of the analyzed solution is caused by colored pigments such as chlorophyll, carotenoids, and xanthophylls. These interferences, which occur especially in herbal drugs consisting of leaves, can be removed by extraction with hexane, though with a considerable loss of flavonoids (27). For plants with a high content of C-glycosides, such as Crataegus, this spectrophotometric method is still not suitable, because C-glycosides can not be hydrolyzed by acid and, therefore, are not extracted with ethyl acetate and not determined.…”