The purpose of this study was to analyze creative thinking skills in the use of local materials as reagents in qualitative biochemical practices. The use of local material reagents can improve students’ creative thinking skills. This research method is quasi-experimental design with pre-test post-test group design. The research instrument consisted of pre-test and post-test questions using tests of creative thinking skills in the form of descriptions and student questionnaires. Data analysis was performed using the SPSS 22.0 program and the percentage of student responses to the practicum was carried out. The results showed that the average claim score of the experimental group was 59.95 and the post-test score was 80.00 with N_Gain 0.50 in the moderate category. In the control group the average pretest score was 38.70 and the posttest score was 44.80 with the lowest category N_Gain 0.09. Further analysis was carried out on each indicator of creative thinking skills which reached 23.16% fluency, 40.28% flexibility, 17.50% originality and 19.06% elaboration. It can be concluded that the use of local materials as reagents in the project-based qualitative biochemical analysis laboratory can improve students’ creative thinking skills, especially in terms of originality and elaboration.
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