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The occurrence of alternative structures for the lithium, sodium, and potassium salts of poly(dG-dC) was determined as a function of hydration using IR spectra of nonoriented gels. Poly(dG-dC).K with added KCl (r = 0.56 where r is the moles of KCl per mole of nucleotide residue) gave results essentially identical to the much studied poly(dG-dC).Na with added NaCl (r = 0.56). Both gave a sharp transition from a unique B structure (hereafter designated B*) to the Z structure upon dehydration. Poly(dG-dC).Li with added LiCl (r = 0.36) assumed the B* structure at high hydration but made a broad transition to the C structures as hydration was lowered. We believe this is the first clear evidence of the C structure for poly(dG-dC). No other structures (A, D, or Z) were observed at any hydration in nonoriented gels. Poly(dG-dC).Na with added ZnCl2 (r = 0.2) existed as a mixture of the B* and Z structures in maximally hydrated gels. A broad, incomplete transition to a higher mole fraction of Z structure occurred upon dehydration. Zn2+ promotes the Z structure for poly(dG-dC) and appears to bind to guanine residues. Poly(dG-dC).Na with added MgCl2 or CaCl2 (r = 0.2) assumed the normal B* structure at maximum hydration with no hint of Z structure. Slight dehydration produced a very sharp transition to the Z structure. Both Mg2+ and Ca2+ are strong promoters of the Z structure but do not bind to cytosine or guanine residues.

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