<b>Hydration of Deoxyribonucleic Acid. III. A Spectroscopic Study of the Effect of Hydration on the Structure of Deoxyribonucleic Acid</b>

Falk, Michael; Hartman, K.A.; Lord, R. C.

doi:10.1021/ja00887a005

Cited by 207 publications

(92 citation statements)

References 2 publications

(5 reference statements)

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“…Soon after its publication, the P-DNA structure appeared to be untenable in the light of the Watson-Crick model (28) for DNA under physiological conditions. Subsequently, some evidence existed to assume the presence of double-stranded P-DNA, but only under very particular conditions in dry DNA (29) or ethanol solutions (30) (see also ref. 31).…”

mentioning

confidence: 99%

On structural transitions, thermodynamic equilibrium, and the phase diagram of DNA and RNA duplexes under torque and tension

Wereszczynski

Andricioaei

2006

Proc. Natl. Acad. Sci. U.S.A.

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A precise understanding of the flexibility of double stranded nucleic acids and the nature of their deformed conformations induced by external forces is important for a wide range of biological processes including transcriptional regulation, supercoil and catenane removal, and site-specific recombination. We present, at atomic resolution, a simulation of the dynamics involved in the transitions from B-DNA and A-RNA to Pauling (P) forms and to denatured states driven by application of external torque and tension. We then calculate the free energy profile along a B-to P-transition coordinate and from it, compute a reversible pathway, i.e., an isotherm of tension and torque pairs required to maintain P-DNA in equilibrium. The reversible isotherm maps correctly onto a phase diagram derived from single molecule experiments, and yields values of elongation, twist, and twist-stretch coupling in agreement with measured values. We also show that configurational entropy compensates significantly for the large electrostatic energy increase due to closer-packed P backbones. A similar set of simulations applied to RNA are used to predict a novel structure, P-RNA, with its associated free energy, equilibrium tension, torque and structural parameters, and to assign the location, on the phase-diagram,

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mentioning

confidence: 99%

On structural transitions, thermodynamic equilibrium, and the phase diagram of DNA and RNA duplexes under torque and tension

Wereszczynski

Andricioaei

2006

Proc. Natl. Acad. Sci. U.S.A.

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“…The possible effects of drying which may be important are damage to macromolecules and membrane leakage. The rise in electrolyte concentration on drying may cause damage to some proteins (Mazur, 1968) but the most potentially damaging effect is the removal of water integral to the structure of macromolecules: DNA (Falk, Hartman and Lord, 1963), collagen (Berendsen, 1962), chitin (Rudall, 1963) and proteins (Bernal, 1965). It has been suggested that high sugar and polyol concentrations may be protective in such cases of drastic water loss (Parker, 1968;Mazur, 1968;Webb, 1965) •.…”

Section: Recovery Of Co2 Fixationmentioning

confidence: 99%

ECOLOGICAL PHYSIOLOGY OF THE LICHEN HYPOGYMNIA PHYSODES

1976

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SUMMARY When air‐dry thalli of Hypogymnia physodes are immersed in water, four main features characterize the rewetting phase. (1) There is an immediate and substantial release of gas, the‘wetting burst’. Although the released gas appears to be mostly CO2, it is non‐metabolic in origin and probably the result of a previous adsorption of gas onto polysaccharide sheaths surrounding fungal hyphae. (2) There is a period of 1‐2 h increased respiration,‘resaturation respiration’, which, unlike basal respiration, is cyanide‐sensitive. The cause of resaturation respiration remains obscure, but could result from exposure of respiratory substrate to enzymes from which they are normally separated. (3) There is a brief period (1–2 min) in which there is a rapid loss of organic and inorganic solutes from the thallus. Since water is a structural component of membranes, removal of some of this water during drying probably caused increased permeability so accounting for the losses observed. (4) There is a very rapid recovery of processes including photosynthesis and nutrient uptake. Even 10 s after rewetting, incorporation of 32P was 60% inhibited by DNP. The ecological significance of these features is discussed, particularly in relation to the apparent need of many lichens for periodic drying.

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“…The purity and molecular integrity of the samples were checked by spectroscopic means (7)(8)(9). Films of 5 to 10 pm thickness were prepared by spreading about 3 mg of dry DNA on an i.r.-transparent window of silver chloride or calcium fluoride, adding a drop of distilled water, and allowing the DNA to form a gel covering about I cm x 2 cm.…”

Section: Dna Filmsmentioning

confidence: 99%

Infrared study of the state of water in the hydration shell of DNA

Falk¹,

Poole²,

Goymour³

1970

Can. J. Chem.

132

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The state of water in the hydration shell of DNA was studied by infrared spectroscopy. The stretching bands of isotopically dilute HDO adsorbed on DNA have nearly the same band profiles as those of HDO in liquid water. This indicates a distribution of hydrogen-bond strengths similar to that in liquid water. At low temperatures, the spectra show that an inner layer of about 10 water molecules per nucleotide is incapable of crystallization, even when the surrounding water crystallizes into ice I. The biopolymer hydration shells are not "ice-like" in the sense of crystalline ordering into an ice-like structure.

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Hydration of Deoxyribonucleic Acid. III. A Spectroscopic Study of the Effect of Hydration on the Structure of Deoxyribonucleic Acid

Cited by 207 publications

References 2 publications

On structural transitions, thermodynamic equilibrium, and the phase diagram of DNA and RNA duplexes under torque and tension

On structural transitions, thermodynamic equilibrium, and the phase diagram of DNA and RNA duplexes under torque and tension

ECOLOGICAL PHYSIOLOGY OF THE LICHEN HYPOGYMNIA PHYSODES

Infrared study of the state of water in the hydration shell of DNA

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