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Insulin receptor substrate-2 (IRS-2) plays critical role in the regulation of various metabolic processes by insulin and IGF-1. The defects in its expression and/or function are linked to diseases like polycystic ovary syndrome (PCOS), insulin resistance and cancer. To predict the transcription factors (TFs) responsible for the regulation of human IRS-2 gene expression, the transcription factor binding sites (TFBS) and the corresponding TFs were investigated by analysis of IRS-2 promoter sequence using MatInspector Genomatix software (Cartharius et al., 2005 [1]). The ibid data is part of author׳s publication (Anjali et al., 2015 [2]) that explains Follicle stimulating hormone (FSH) mediated IRS-2 promoter activation in human granulosa cells and its importance in the pathophysiology of PCOS. Further analysis was carried out for binary interactions of TF regulatory genes in IRS-2 network using Cytoscape software tool and R-code. In this manuscript, we describe the methodology used for the identification of TFBSs in human IRS-2 promoter region and provide details on experimental procedures, analysis method, validation of data and also the raw files. The purpose of this article is to provide the data on all TFBSs in the promoter region of human IRS-2 gene as it has the potential for prediction of the regulation of IRS-2 gene in normal or diseased cells from patients with metabolic disorders and cancer.

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Background: Polycystic ovary syndrome (PCOS) is a growing morbidity in young women globally. This disease has an association with several exogenous factors like irregularity of menses, hirsutism and obesity. Very few standardized self-assessment tools based on easily observable factors are available for use in the Indian population, which can help them to assess their PCOS risk accurately.Methods: Undergraduate women of the age group 18-22 years enrolled in a university campus participated in the survey questionnaire. Nineteen questions with binary answers as “yes” or “no” were used for self-assessment test. Each “yes” was scored as one mark, and each “no” scored as zero, leading to the maximum score of 19. Scores of the women with irregular menses (test group) were compared to those of regular menses (control group). Welch’s corrected t-test was used to calculate the significance at 5% between the groups. The clinical assessment confirmed the presence or absence of PCOS condition.Results: One thousand and fifty-four women participated in the study. The study showed that 262 (24.8%) of young women reported irregular menstrual cycle. The average total score of the control group was 3.07±2.35, whereas that of the women with irregular menses was 5.93±2.86. 21 out of 28 participants, who scored high, were diagnosed with PCOS, on clinical assessment by Rotterdam criteria.Conclusions: The self-assessment test can assess the risk of PCOS. This test has 75% sensitivity and accuracy in predicting the presence of PCOS.

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A BSTRACT Background: Several symptoms are typically experienced after a coronavirus disease 2019 (COVID-19) infection. Worldwide, a lot of women are reporting irregularities in their menstrual cycles post-COVID-19 infection. The purpose of this study is to investigate the prevalence of menstrual pattern among young girls during the second wave of COVID-19 pandemic and to determine the risk factors related to lifestyle among young girls. Methods: A cross-sectional study was conducted using a self-designed questionnaire encompassing details of the menstrual pattern, features of hyperandrogenism, lifestyle, and comorbidity among young girls aged 16-24 years. Results: The data from 508 girls fulfilling the inclusion criteria were analyzed. The prevalence of irregular menstrual cycle was found to be 29.1%. Further analysis revealed that a significant percentage of girls with irregular menstrual cycle suffer from depression (14.9%) and are often staying stressed (40.5%) in comparison to the girls having regular menstrual cycle. Also, a total of 58 girls out of 508 were diagnosed with polycystic ovary syndrome (PCOS). Among various comorbid conditions, obesity was found in 60% of girls having PCOS followed by an eating disorder. Conclusions: A significant increase in irregular menstrual cycle in young girls was found during the second wave of COVID-19. The risk factors for causing the irregular menstrual cycle were found to be insomnia, stress, and depression

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Insulin receptor signalling receptor regulates the process of follicular development and maturation, however the regulation of insulin receptor (IR) and its signalling component during folliculogenesis is not well understood. This study demonstrates FSH mediated regulation of insulin receptor isoforms specially IR-B, its substrates and some of the insulin related responses like glycogen synthesis and cell proliferation. There was significant increase in mRNAs expression for insulin receptor (IR) isoforms, IR-A and IR-B in ovary in response to PMSG and in granulosa cells (GCs) in response to FSH. IR protein increased in GCs in response to FSH in a dose and time dependent manner. Interestingly, the expression of mRNAs and proteins for IRS-1 and IRS-2 increased significantly in GCs by FSH. Serine phosphorylation (Ser 636/639) of IRS-1 was decreased by FSH, thus facilitating IRS-1 activation. FSH stimulated glycogen synthesis in a dose dependent manner both by PI 3 kinase dependent and independent pathways. Insulin regulated the amount of FSH stimulated glycogen synthesis by granulosa cells. In contrast, FSH and insulin synergistically stimulated glycogen synthesis GC proliferation which was completely inhibited by LY294002. Knockdown of IRS-1 mRNA by siRNA inhibited FSH stimulated GC proliferation indicating an important role of IRS-1 downstream of FSH. Further research is required to delineate the signalling components involving IRS-1 and IRS-2 in response to FSH and thus involved in the cross-talk between FSH and insulin in GCs. Results thus demonstrate that pituitary FSH regulates insulin receptor and its substrates in rat GCs which might be important for follicular growth and oocyte development.