Amperometric glucose biosensors were constructed by codeposition of glucose oxidase (GOx) with chitosangold (chitosan-AuNP) on gold-Prussian Blue (Au-PB) nanoparticles modified glassy carbon electrodes (GCE). The high stability of the biosensors was achieved using electrochemically controllable in situ Au-PB film preparation following the film covering with GOx layer encrusted with chitosan-AuNP. The biosensors showed 95 % response time up to 10 s, sensitivity up to 9.5 A M -1 cm -2 and linear calibration graph up to 3 mM of glucose.
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