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Osteocytes respond to dynamic fluid shear loading by activating various biochemical pathways, mediating a dynamic process of bone formation and resorption. Whole-cell deformation and regional deformation of the cytoskeleton may be able to directly regulate this process. Attempts to image cellular deformation by conventional microscopy techniques have been hindered by low temporal or spatial resolution. In this study, we developed a quasi-three-dimensional microscopy technique that enabled us to simultaneously visualize an osteocyte's traditional bottom-view profile and a side-view profile at high temporal resolution. Quantitative analysis of the plasma membrane and either the intracellular actin or microtubule (MT) cytoskeletal networks provided characterization of their deformations over time. Although no volumetric dilatation of the whole cell was observed under flow, both the actin and MT networks experienced primarily tensile strains in all measured strain components. Regional heterogeneity in the strain field of normal strains was observed in the actin networks, especially in the leading edge to flow, but not in the MT networks. In contrast, side-view shear strains exhibited similar subcellular distribution patterns in both networks. Disruption of MT networks caused actin normal strains to decrease, whereas actin disruption had little effect on the MT network strains, highlighting the networks' mechanical interactions in osteocytes.

Waterlogging is an important constraint of global production of rape. The effects of seed film coating with 0.0075% uniconazole on the growth and physiology was investigated using seedlings from three varieties of rape (Brassica napus L.) subjected to waterlogging. While seed coating with uniconazole had no significant effect on germination percentage during waterlogging stress, it enhanced root vigour, increased root length, root volume and root dry weight. It also significantly enhanced leaf dry weight and ratio of root to shoot, but induced a significant decrease in shoot height and stem dry weight. Seed film coating with uniconazole also significantly increased the activities of the antioxidant enzymes, POD, CAT and SOD, and soluble sugar concentration during waterlogging. Thus, it suggests that seed film coating with uniconazole at a suitable concentration can improve rape seedling growth and increase seedling establishment during waterlogging.

Long-term streamflow forecast is of great significance for water resource application and management. However, accurate monthly streamflow forecasting is challenging due to its non-stationarity and uncertainty. Time series analysis methods have been proved to perform well in stationary time series forecasting, which can be derived from decomposition of the non-stationary sequence. As common decomposition methods in time domain, Empirical Mode Decomposition (EMD) and Ensemble Empirical Mode Decomposition (EEMD) are selected to decompose the components with different time-scale characteristics in the original hydrological time series in this study. The derived components are proved to be stationary by the stationarity test. Thus, Autoregressive Integrated Moving Average (ARIMA) model, a simple and effective time series analysis method, is used to forecast the components. A hybrid EMD/EEMD-ARIMA model is proposed in this study for long-term streamflow forecasting, which is applied to the upper stream of the Yellow River. The original daily streamflow time series of six years at the Tangnaihai station are firstly decomposed by EMD/EEMD into several stationary or simple non-stationary sub-series to explore detailed data information with different time scales. ARIMA models with appropriate parameters are then established for each subsequence to forecast the stream flow of the next year. Predicted ten-day and monthly stream flow is finally obtained combing the predictions of all the components. The EMD-ARIMA hybrid model performs best in forecasting high and moderate value of streamflow and fits best with the observation compared with EEMD-ARIMA and ARIMA models. The results not only verify the effectiveness of the proposed hybrid EMD/EEMD-ARIMA model in exploiting comprehensive information to improve the prediction but also indicate that the EMD-ARIMA model with end points disposal performs the best and can be used for long-term hydrological forecasting.

Hydropower can be an ideal compensation for fluctuant photovoltaic (PV) power due to its flexibility. In this study, a multiobjective optimization model considering energy generation and consumption simultaneously for a hydro‐PV hybrid power system is proposed. With the daily mean radiation intensity and temperature, the PV power output is calculated. Taking reservoir release as the decision variable, the total energy generation of the hydro‐PV system is maximized. Meanwhile, the gap between the energy generation and the energy consumption is minimized to reduce the abandoned PV power or hydropower. The proposed multiobjective model is optimized by Non‐dominated Sorting Genetic Algorithms‐II (NSGA‐II). The Longyangxia Project, the largest hydro‐photovoltaic hybrid power system in the world is taken as the study case to estimate the optimal operational strategies for different objectives in wet year, normal year, and dry year, respectively. The optimal operation process of the reservoir is presented, which is instructive for the operation in the future.

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