The aim of the study was to determine the level of insecticide resistance and diversity in Anopheles mosquitoes in northern Uganda. Standard WHO insecticide susceptibility test assays were used to test for susceptibility to 0.5% malathion, 0.1% bendiocarb, 0.05% deltamethrin and 0.75% permethrin on 3-5 day old generation one progeny. We also screened for species diversity and knockdown resistance using PCR assay. Results: Anopheles gambiae s.s. is the predominant malaria vector in northern Uganda followed by An. arabiensis. An. gambiae s.s. was susceptible to malathion and bendiocarb with the observed mortality rate of 100% and 98-100% observed respectively while very high resistance was observed with deltamethrin and permethrin. Minimal KDReastern variant homozygous forms of 8.3% in An. gambiae s.s. were detected in Oyam district. In conclusion, this study confirms that An. gambiae s.s. females are susceptible to malathion and bendiocarb while high intensity of resistance was observed with deltamethrin and permethrin in the same area. Use of carbamate and organophosphate insecticides bendiocarb and malathion for indoor residual spraying activities in northern Uganda is highly recommended since high levels of pyrethroids resistance (deltamethrin and permethrin) was detected in the area.

Objective The aim of the study was to determine the level of insecticide resistance and diversity in Anopheles mosquitoes in northern Uganda. Standard WHO insecticide susceptibility test assays were used to test for susceptibility to 0.5% malathion, 0.1% bendiocarb, 0.05% deltamethrin and 0.75% permethrin on 3–5 day old generation one progeny. We also screened for species diversity and knockdown resistance using PCR assay.Results Anopheles gambiae s.s is the predominant malaria vector in northern Uganda followed by An. arabiensis. An. gambiae s.s. was susceptible to malathion and bendiocarb with the observed mortality rate of 100% and 98–100% observed respectively while very high resistance was observed with deltamethrin and permethrin. Minimal KDR-eastern variant homozygous forms of 8.3% in An. gambiae s.s were detected in Oyam district. In conclusion, this study confirms that An. gambiae s.s. females are susceptible to malathion and bendiocarb while high intensity of resistance was observed with deltamethrin and permethrin in the same area. Use of carbamate and organophosphate insecticides bendiocarb and malathion for indoor residual spraying activities in northern Uganda is highly recommended since high levels of pyrethroids resistance (deltamethrin & permethrin) was detected in the area.

Background: Karamoja region of Uganda previously classified as low malaria transmission zone is currently experiencing significant upsurge of malaria incidences. Long lasting insecticidal nets (LLINs) impregnated with pyrethroids constitute a major tool for malaria control in this region. Efficacy of this tool can be hampered by resistance to the pyrethroids in the Anopheles mosquito vectors. Resistance status of these mosquitoes in this region is poorly understood, effectively hampering better understanding of the impact of LLINs in the malaria control initiative. Here, we assessed susceptibility of the Anopheles arabiensis from the region to deltamethrin, permethrin (pyrethroids) and pirirmiphos-methyl (organophosphate) insecticides.Method: We collected anopheline mosquito larvae from their natural habitats and reared them to adult emergence in situ field insectary in Karamoja region. We then identified them morphological to species level and exposed 513 emerge adult female An gambiae s.l., mosquitoes to diagnostic dosages of deltamethrin (0.05%), permethrin (0.75%) and pirimiphos-methyl (0.25%) pyrethroids exposure using the standard WHO insecticide susceptibility test assay. Synergic assays using piperonyl butoxide (PBO) were done to check for the involvement of detoxification enzymes in pyrethroid resistant populations. We then screened for knockdown resistance (KDR) and mosquito species diversity using Polymerase Chain Reaction (PCR).Results: Majority (96%) of the mosquitoes we sampled were identified as An. arabiensis and 4% as An. gambiae sensu stricto. We observed cross-resistance to both deltamethrin (11.9%) and permethrin (47%) but susceptibility (100% mortality) to pirimiphos-methyl in An. arabiensis. The pre-exposure to PBO ameliorated the resistance to both pyrethroids. We detected homozygous KDR -eastern variant in 1.8 and 50% of the An. arabiensis and An. gambiae s.s. respectively.Conclusion: Anopheles arabiensis and An. gambiae s.s. are the malaria vector in Karamoja region with An. arabiensis predominating. Both species are susceptible to pirimiphos-methyl but resistant to both deltamethrin and permethrin, through a metabolic process (phenotype). Mosquotoes with genetic (kdr) mutations for resistance were minimal and hence have minimal contribution to the pyrethroid resistance profile. An. arabiensis can thus be controled in Karamoja region using deltamethrin and/or permethrin impregnated mosquito nets integrated with PBO and/or through indoor residual spraying of sprayable human dwellings with pirimiphos-methyl.

Background Karamoja region of Uganda previously classified as low malaria transmission zone is currently experiencing significant upsurge of malaria incidences. Long lasting insecticidal nets (LLINs) impregnated with pyrethroids constitute a major tool for malaria control in this region. Efficacy of this tool can be hampered by resistance to the pyrethroids in the Anopheles mosquito vectors. Resistance status of these mosquitoes in this region is poorly understood, effectively hampering better understanding of the impact of LLINs in the malaria control initiative. Here, we assessed susceptibility of the Anopheles arabiensis from the region to deltamethrin, permethrin (pyrethroids) and pirirmiphos-methyl (organophosphate) insecticides. Method We collected anopheline mosquito larvae from their natural habitats and reared them to adult emergence in situ field insectary in Karamoja region. We then identified them morphological to species level and exposed 513 emerge adult female An gambiae s.l., mosquitoes to diagnostic dosages of deltamethrin (0.05%), permethrin (0.75%) andpirimiphos-methyl (0.25%) pyrethroids exposure using the standard WHO insecticide susceptibility test assay. Synergic assays using piperonyl butoxide (PBO) were done to check for the involvement of detoxification enzymes in pyrethroid resistant populations. We then screened for knockdown resistance (KDR) and mosquito species diversity using Polymerase Chain Reaction (PCR). Results Majority (96%) of the mosquitoes we sampled were identified as An. arabiensis and 4% as An. gambiae sensu stricto . We observed cross-resistance to both deltamethrin (11.9%) and permethrin (47%) but susceptibility (100% mortality) to pirimiphos-methyl in An. arabiensis . The pre-exposure to PBO ameliorated the resistance to both pyrethroids. We detected homozygous KDR -eastern variant in 1.8 and 50% of the An. arabiensis and An. gambiae s.s. respectively. Conclusion Anopheles arabiensis and An. gambiae s.s. are the malaria vector in Karamoja region with An. arabiensis predominating.Both species are susceptible to pirimiphos-methyl but resistant to both deltamethrin and permethrin, through a metabolic process (phenotype). Mosquotoes with genetic (kdr) mutations for resistance were minimal and hence have minimal contribution to the pyrethroid resistance profile. An. arabiensis can thus be controled in Karamoja region using deltamethrin and/or permethrin impregnated mosquito nets integrated with PBO and/or through indoor residual spraying of sprayable human

Background. Malaria remains one of the most critical disease causing morbidity and mortality in Uganda. Indoor residual spraying (IRS) and the use of insecticide-treated bed nets are currently the predominant malaria vector control interventions. However, the emergence and spread of insecticide resistance among malaria vectors threaten the continued effectiveness of these interventions to control the disease, particularly in high transmission areas. To inform decisions on vector control, the current study evaluated the Anopheles malaria vector species and their susceptibility levels to 0.1% bendiocarb and 0.25% pirimiphos-methyl insecticides used in IRS intervention program in Namutumba district, Eastern Uganda. Methods.Anopheles larvae were collected between March and May 2017 from different breeding sites in the parishes of Nsinze and Nawaikona in Nsinze sub-county and reared to adults to assess the susceptibility status of populations in the study area. Mosquitoes were identified using morphological keys and species-specific polymerase chain reaction (PCR) assays. Susceptibility tests were conducted on 2- to 5-day-old non-blood-fed adult female Anopheles that emerged using insecticide-impregnated papers with 0.1% bendiocarb and 0.25% pirimiphos-methyl following standard World Health Organization (WHO) insecticide susceptibility bioassays. A Log-probit regression model was used to derive the knock-down rates for 50% and 95% of exposed mosquitoes. Results. A total of 700 mosquito larvae were collected from different breeding sites. Morphological identification showed that 500 individuals that emerged belonged to Anopheles gambiae sensu lato (s.l.), the main malaria vector. The PCR results showed that the dominant sibling species under the A. gambiae complex was Anopheles arabiensis 99.5% (395/397). WHO bioassay tests revealed that the population of mosquitoes exhibited high levels of susceptibility (24-hour post-exposure mortality 98–100%) to both insecticides tested. The median knock-down time, KDT50, ranged from 6.6 to 81.4 minutes, while the KDT95 ranged from 21.6 to 118.9 minutes for 0.25% pirimiphos-methyl. The KDT50 for 0.1% bendiocarb ranged from 2.8 to 62.9 minutes, whereas the KDT95 ranged from 36.0 to 88.5 minutes. Conclusions. These findings indicate that bendiocarb and pirimiphos-methyl are still effective against the major malaria vector, A. arabiensis in Nsinze sub-county, Namutumba district, Uganda and can be effectively used for IRS. The study has provided baseline information on the insecticide susceptibility status on malaria vectors in the study area. However, routine continuous monitoring program of insecticide susceptibility and malaria vector composition is required so as to guide future decisions on insecticide use for IRS intervention toward malaria elimination and to track future changes in vector population.

Objective: The aim of the study was to determine the level of insecticide resistance and diversity in Anopheles mosquitoes in northern Uganda. Standard WHO insecticide susceptibility test assays were used to test for susceptibility to 0.5% malathion, 0.1% bendiocarb, 0.05% deltamethrin and 0.75% permethrin on 3-5 day old generation one progeny. We also screened for species diversity and knockdown resistance using PCR assay.Results: Anopheles gambiae s.s. is the predominant malaria vector in northern Uganda followed by An. arabiensis. An. gambiae s.s. was susceptible to malathion and bendiocarb with the observed mortality rate of 100% and 98-100% observed respectively while very high resistance was observed with deltamethrin and permethrin. Minimal KDR-eastern variant homozygous forms of 8.3% in An. gambiae s.s. were detected in Oyam district. In conclusion, this study confirms that An. gambiae s.s. females are susceptible to malathion and bendiocarb while high intensity of resistance was observed with deltamethrin and permethrin in the same area. Use of carbamate and organophosphate insecticides bendiocarb and malathion for indoor residual spraying activities in northern Uganda is highly recommended since high levels of pyrethroids resistance (deltamethrin & permethrin) was detected in the area.

Objective: The aim of the study was to determine the level of insecticide resistance and diversity in Anopheles mosquitoes in northern Uganda. Standard WHO insecticide susceptibility test assays were used to test for susceptibility to 0.5% malathion, 0.1% bendiocarb, 0.05% deltamethrin and 0.75% permethrin on 3-5 day old generation one progeny. We also screened for species diversity and knockdown resistance using PCR assay.Results: Anopheles gambiae s.s is the predominant malaria vector in northern Uganda followed by An. arabiensis. An. gambiae s.s. was susceptible to malathion and bendiocarb with the observed mortality rate of 100% and 98-100% observed respectively while very high resistance was observed with deltamethrin and permethrin. Minimal KDR-eastern variant homozygous forms of 8.3% in An. gambiae s.s were detected in Oyam district. In conclusion, this study confirms that An. gambiae s.s. females are susceptible to malathion and bendiocarb while high intensity of resistance was observed with deltamethrin and permethrin in the same area. Use of carbamate and organophosphate insecticides bendiocarb and malathion for indoor residual spraying activities in northern Uganda is highly recommended since high levels of pyrethroids resistance (deltamethrin & permethrin) was detected in the area.

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