Edible insects have been suggested as an alternative and sustainable source of fats, proteins and vitamins for humans. However, their nutritional content may largely depend on the diets offered for insects. Diet-based manipulation of fatty acids in edible insects shows promise to possibly increase their essential and long-chained polyunsaturated fatty acid content and thus further enhance human diets. We reared the edible grasshopper, Ruspolia differens, under various diets with manipulated content of fatty acids, proteins and carbohydrates. The manipulated diets dramatically altered the insects’ content and composition of fatty acids, especially the content of polyunsaturated fatty acids. Dietary fatty acids were stored largely unaltered in insects. Artificial diets with high content of linoleic, α-linolenic, eicosapentaenoic or docosahexaenoic acids, increased the content of these fatty acids in the insect tenfold. The manipulated diets also affected the omega-6/omega-3 (n-6/n-3) fatty acid ratio and developmental performance. Lack of protein and fats in the diet prolongs the development and leads to lower final weight. To achieve required n-6/n-3 ratio for human consumption and to maximise biomass gain and weight, diverse diets with a source of n-3 fatty acids and protein should be used when rearing R. differens. These results demonstrate the potential for improving human nutrition in an effective and sustainable way by producing edible insects with designed nutritional content.

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Background Climate change poses a threat to the sustainability of food production among small-scale rural communities in Sub-Saharan Africa that are dependent on rain-fed agriculture. Understanding farmers’ adaptations and the determinants of their adaptation strategies is crucial in designing realistic strategies and policies for agricultural development and food security. The main objectives of this study were to identify the adaptation strategies used by smallholder farmers to counter the perceived negative effects of climate change in northern Uganda, and factors influencing the use of specific adaptation strategies. A cross-sectional survey research design was employed to collect data from 395 randomly selected smallholder farmers’ household heads across two districts by the administration of a semi-structured questionnaire. Binary logistic regression was used to analyze the factors influencing farmers’ adaptation to climate change. Results The three most widely practiced adaptation strategies were planting of different crop varieties, planting drought-resistant varieties, and fallowing. Results of the binary logit regression model revealed that marital status of household head, access to credit, access to extension services, and farm income influenced farmers’ adoption of planting drought-resistant varieties as an adaptation strategy while access to credit, annual farm income, and time taken to market influenced adoption of planting improved seeds. Gender of household head and farm income had a positive influence on farmers’ adoption of fertilizer and pesticide use. Farming experience, farm income, and access to extension services and credit influenced farmers’ adoption of tree planting. Household size, farming experience, and time taken to market had positive influence on the use of fallowing, while size of land cultivated significantly influenced farmers’ planting of different crop varieties as an adaptation strategy. Conclusion Findings of the study suggest there are several factors that work together to influence adoption of specific adaptation strategies by smallholder farmers. This therefore calls for more effort from government to strengthen the provision of agricultural extension services by improving its climate information system, providing recommended agricultural inputs and training farmers on best agronomic practices to enhance their holistic adaptation to the effect of climate change.

Background: Diarrhoea remains a major cause of morbidity and mortality in children under 5 years in sub-Saharan Africa. Of the three East African countries, Uganda has the worst mortality rate in children < 5 years, with 22% of these deaths attributed to diarrhoea. For proper planning and implementation of control, an understanding of the prevalence and determinants of the disease is crucial. This study assessed the prevalence of diarrhoea and related risk factors among children < 5 years in Pajule Subcounty, Pader District in northern Uganda. Methods: A cross-sectional survey was conducted in April 2018, covering 244 randomly selected households having children < 5 years old in the study area. A semi-structured questionnaire was used to interview the households about diarrhoeal history in their children in the last 2 weeks preceding the survey, and on the risk factors predisposing children to diarrhoeal infections. Bivariate and multivariate logistic regression analyses with a 95% confidence interval and p < 0.05 was used to identify the risk factors associated with childhood diarrhoeal disease. Results: We found a prevalence of diarrhoea of 29.1% [95% CI (23.7-35.0)] among children < 5 years in Pajule Subcounty during the 2 weeks preceding the survey. Use of unprotected water sources, age of child caretaker, child weaning time and family size had significant associations with diarrhoeal morbidity. Conclusion: The prevalence of childhood diarrhoea among children < 5 years of age in rural settings of Pajule Subcounty was higher than the Ugandan national average. Use of unprotected water sources, age of child caretaker, child weaning time and family size were identified as predictors of diarrhoeal occurrence. These findings underscore the need for improving access to clean water and providing community health education as the best methods for fighting childhood diarrhoea in the study area.

Cowpea is an important legume crop widely grown in sub-Saharan Africa for food and feed. However, it is largely challenged by bruchid, a serious storage pest resulting in losses in quantity and quality of grains. Therefore, this research was designed to contribute to the breeding of cowpea resistance to bruchid through the identification of candidate genes associated with resistance to bruchid. A total of 217 mini-core cowpea accessions were genotyped and phenotyped for their reactions to bruchid. To determine the genomic regions linked with bruchid resistance, 41,948 polymorphic SNP markers were used. Genome-wide association study identified 11 SNPs linked to the average number of eggs, holes, insect emergence and development period and Dobie susceptibility index. Gene search via Phytozome identified six candidate genes (

Ruspolia differens (Serville) (Orthoptera: Tettigoniidae, Conocephalinae) (its common names including ‘African edible bush‐cricket’, ‘edible grasshopper’, and ‘nsenene’) is an important source of food for humans in East Africa, but its ecology and biology are poorly understood. We explored the host plants of R. differens with a series of no‐choice and multiple‐choice laboratory experiments using 18 local common grass and sedge species in Uganda. In no‐choice experiments, the degree of acceptance differed significantly among the studied plant species, but in only three species were leaves rejected and in one species were inflorescences rejected. The pattern of acceptance among plant species was different in the local vs. swarming populations. Leaves were generally more accepted by the local population, whereas inflorescences were generally more accepted by the swarming population. Both leaves and inflorescences were more readily accepted by males than by females. According to the multiple‐choice experiments, R. differens preferred inflorescences over leaves. Our results demonstrate that R. differens is a facultatively oligophagous grass‐specialist, which has a clear preference for certain grass or sedge species (especially inflorescences), but it accepts many host plants if the preferred ones are not available. To preserve viable natural populations of R. differens in East Africa in the long term, our results draw special attention to the availability of grasslands where accepted and preferred host plants are available year‐round.

Background: Diarrhoea remains a major cause of morbidity and mortality in children < 5 years in sub-Saharan Africa. Uganda has the worst mortality rate in children < 5 years among the three East African countries, with 22% of these deaths attributed to diarrhoea. For proper planning and implementation of control interventions, an understanding of the prevalence and determinants of the disease is crucial. This study assessed the prevalence of diarrhoea and related risk factors among children aged < 5 years in Pajule sub-county in Pader district in northern Uganda. Methods: A cross-sectional survey was conducted covering 244 randomly selected households having children < 5 years old in the study area. A semi-structured questionnaire was used to interview the households about diarrhoeal history in their children in the last two weeks preceding the survey, and on the risk factors predisposing children to diarrhoeal infections. Descriptive statistics was used to summarize the risk factors whereas bivariate and multivariate logistic regression analyses (95% confidence interval and p < 0.05) were used to identify the risk factors associated with childhood diarrhoea.Results: We found a prevalence of diarrhoea of 29.1% among children < 5 years of age in Pajule sub-county during the two weeks preceding the survey. Multivariate logistic regression revealed significant associations between diarrhoeal prevalence and use of unprotected water sources (adjusted Odd Ratio, aOR=2.866, 95% CI: [1.431 – 5.741]), presence of animals in respondents’ homes (aOR=3.950, 95%CI: [1.399 – 11.156]) and infrequent hand washing practices (aOR=2.737, 95% CI: [1.304 – 5.743]). Conclusion: The present study identified a high prevalence of diarrhoea among children < 5 years of age in Pajule sub-county, higher than the Ugandan national average. Extensive use of unprotected water sources, animals’ presence in homes and poor hand washing practices had significant associations with diarrhoeal occurrence. These findings underscore the need for continuous community health promotion emphasizing good hand washing practices and interventions that target improvement of hygiene practices at homes as the best methods for fighting childhood diarrhoea in the study area.

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