The combination of medroxyprogesterone acetate (MPA) and gonadotropin chorionic (eCG) has been widely used to synchronize oestrus cycle in sheep, but their effects on the gene expression in uterine tissue are yet to be elucidated. To evaluate the effect of MPA + eCG or prostaglandin analogue (PA) treatments on the rate of oestrus cycle synchronization, as well as further hormone production and gene expression profiles in uterine tissue, 14 Santa Inês ewes were randomly selected. The MPA + eCG group (n=7) received intravaginal insertion of MPA-impregnated sponges for 14 days and was administered 350 IU eCG on the day of sponge withdrawal. The PA group (n=7) was administered two doses of 100 μg of PA separated by 12 days. The ewes were assessed for the rate of oestrus cycle synchronization and the serum concentrations of progesterone (P4) and estradiol (E2). Additionally, the expression of estrogen receptor (ERα), progesterone receptor (P4R), and immunolocalization of interferon receptor (IFNAR1) in the uterine tissue samples collected 15th day post-mating were examined. The rate of oestrus cycle synchronization was 100% (n=7/7) and 57.14% (n=4/7) in the MPA + eCG and PA groups, respectively. Moreover, the MPA + eCG group exhibited higher serum concentration of P4 than the PA group (p < 0.05). However, the E2 serum concentration did not differ between the two groups (p > 0.05). The relative expression of P4R and ERα mRNA analyzed using real-time PCR and immunodetection of IFNAR1 were similar between the two groups tested (p > 0.05). Conclusively, MPA + eCG treatment improved the rate of oestrus cycle synchronization and endogenous P4 production; however, it did not affect the expression of sex steroid receptors and IFNAR1 in uterine ovine tissue.
As perdas gestacionais nas espécies canina e felina podem resultar em reabsorção embrionária; aborto, caracterizado pela expulsão de fetos vivos ou mortos, morte fetal com mumificação ou maceração. O trauma é considerado uma das causas não-infecciosas de mortalidade fetal e neonatal felina, podendo ocorrer de forma acidental. O objetivo deste trabalho foi relatar um caso de autólise fetal ectópica pós-traumática em gata. Foi atendida no Hospital Veterinário Universitário UFPI/CPCE um felino, fêmea, sem padrão de raça definida (SPRD), 2 anos de idade, pensando 3,0 kg com histórico de gestação e que havia sofrido um trauma na região abdominal há 15 dias. Após a realização da anamnese, exame físico, clínico e exames complementares a paciente foi encaminhada ao setor de cirurgia e submetida a celiotomia, na qual constatou-se a presença de um feto em processo de autólise na cavidade abdominal, além de uma lesão em processo de cicatrização no corno uterino esquerdo. Realizou-se a retirada do feto, seguida da ovariosalpingohisterectomia. Conclui-se que a morte fetal ocorreu posteriormente ao processo de ruptura uterina com a passagem do feto para a cavidade abdominal e subsequente autólise, tendo o fator traumático como causa da ruptura.
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