The cultured photobiont Trebouxia sp. of Ramalina celastri was successively extracted at 100 ‡C with hot water, 2% aqueous KOH, and 10% aqueous KOH to give polysaccharide-containing fractions A (2.9%), B (3.9%), and C (0.9% yield) respectively. The intact biont contained 3.8% amylose, which was present in each fraction, and was identified by a blue color formed with iodine solution. In fraction A, and following retrogradation from aqueous solution, it was characterized by 13 C-NMR spectroscopy. Fraction B was treated with Kamylase to give a water-soluble fraction consisting mainly of L-mannose-containing polysaccharides (1.5% yield), whose main component had dn/dc 0.162 and M r 17 kDa. Fraction C was subjected to freeze^thawing and the precipitate was treated with K-amylase to give a resistant, linear, low molecular mass (
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