Aim: To expose the challenges faced by mothers of newborn babies who are submitted to phototherapy treatment in a rooming-in setting. Methods: A descriptive study, using a qualitative approach, conducted by interviewing 10 mothers whose babies experienced neonatal phototherapy while rooming-in between the months of June and October of 2014 in a public health institution in the city of Rio de Janeiro. Bardin’s Content Analysis was used for analysis. Results: Three analytical categories emerged: Mothers’ feelings and reactions towards phototherapy treatment; The lack ofknowledge when facing a new reality and The healthcare team as a support network for the mothers. Discussion: Despite the simplicity of phototherapy treatment, it makes the mothers feel negative emotions such as anguish, sadness and guilt. Conclusion: The nursing staff must be prepared to hear and guide the mothers throughout phototherapy, taking into consideration that it is a negative experience during the newborn hospitalization.
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