Vários procedimentos são utilizados para avaliar o potencial fisiológico de sementes, sendo fundamental a utilização daqueles que reflitam melhor o desempenho em campo. A pesquisa teve como objetivo avaliar o potencial fisiológico de sementes de soja por meio de testes de avaliação rápida, de vigor, de emergência de plântulas em campo e análise computadorizada de imagens (SVIS®), procurando identificar o procedimento mais eficiente na separação de lotes. Cinco lotes das cultivares BRS Valiosa RR e CD 208 foram avaliados em duas épocas (antes e após armazenamento) pelos testes de germinação, de avaliação rápida (embebição em água e pH do exsudato), de vigor (envelhecimento acelerado e condutividade elétrica), de emergência de plântulas em campo e análise computadorizada de imagens (SVIS®). Os testes de germinação em areia, de condutividade elétrica e de embebição em água foram eficientes na separação dos lotes, mas apenas este último possibilitou identificar diferenças consistentes entre os lotes nos dois períodos avaliados. Assim, o teste de embebição em água pode ser considerado promissor na composição de programas de controle de qualidade.
-Vigor tests are important tools for verifying the physiological potential of seed lots; however, various aspects can interfere in the consistency of the test results. The search for less subjective procedures has drawn the interest of researchers. The aim of this study was to adapt the methodology of the controlled deterioration test and verify the efficiency of the Seed Vigor Imaging System (SVIS ® ) to evaluate the vigor of crambe seeds. Initially, seed moisture content was determined and germination and vigor were evaluated in five seed lots. For the controlled deterioration test, the seed moisture content was adjusted to 18%, 20%, and 22%, and the results were interpreted at four and five days after sowing. The controlled deterioration test should be performed with initial adjustment of seed moisture content to 18%, and the germination test should be evaluated on the fifth day after sowing. SVIS ® provided information on the vigor index and the length and uniformity of seedling development. Computerized image analysis is effective in evaluating the vigor of crambe seeds, and the uniformity of seedling development parameter offers the most consistent data.Index terms: Crambe abyssinica, software, seed vigor.Teste de deterioração controlada e uso do sistema automatizado de análise de imagens de plântulas (SVIS®) na avaliação do potencial fisiológico de sementes de crambe RESUMO -Os testes de vigor são ferramentas importantes para aferir o potencial fisiológico de lotes de sementes, porém, vários aspectos podem interferir na consistência dos resultados desses testes, tendo a busca por procedimentos menos subjetivos despertado o interesse dos pesquisadores. Este trabalho foi realizado com o objetivo de adequar a metodologia do teste de deterioração controlada e verificar a eficiência do sistema automatizado de análise de imagens de plântulas (SVIS ® ) na avaliação do vigor de crambe. Inicialmente, cinco lotes de sementes foram submetidos à determinação do teor de água e avaliações de germinação e vigor. Para o teste de deterioração controlada, o teor de água das sementes foi ajustado para 18, 20 e 22%, e a interpretação dos resultados realizada aos quatro e cinco dias após a semeadura. O teste de deterioração controlada deve ser realizado com ajuste inicial do teor de água para 18% e a avaliação da germinação realizada no quinto dia. O SVIS ® forneceu informações sobre o índice de vigor, comprimento e uniformidade de desenvolvimento de plântulas. A análise computadorizada de imagens é eficiente para avaliar o vigor de sementes de crambe, sendo o parâmetro uniformidade de desenvolvimento de plântulas o que apresenta dados mais consistentes.Termos para indexação: Crambe abyssinica, software, vigor de sementes.
Adequate procedures to evaluate seed vigor are important. Regarding the electrical conductivity test (EC), the interference in the test results caused by seed-borne pathogens has not been clarified. This research was carried out to study the influence of Phomopsis sojae and four treatments (control, seeds incubated without fungi, seeds incubated with Phomopsis and seeds incubated with Colletotrichum) were used with eight (5.5 mm large seeds) and six (6.5 mm large seeds) replications. All seeds submitted to PDA medium had their germination reduced in comparison to the control seeds. This reduction was also observed when seed vigor and leached ions were considered. The presence of Phomopsis sojae fungus in soybean seed samples submitted to the EC test may be the cause of misleading results.
Adequate seed vigour tests are fundamental in evaluating vigour level and consequent performance of seed lots in storage and in the field conditions. The accelerated ageing is one of the most important of these tests. To establish the best accelerated ageing procedure for evaluating the vigour of crambe seeds, we compared results from the traditional method (ageing periods of 24, 48, and 72 hours at temperatures of 41 and 45 °C) and the saturated salt accelerated ageing (SSAA) (NaCl) method. In addition to accelerated ageing, germination in a sand substrate, seedling emergence in laboratory, and seed moisture content before and after seed ageing were determined. Our first count was highly sensitive in detecting seed vigour level. Both the traditional and SSAA methods can be used to efficiently classify the vigour of crambe seed lots and could be promising techniques for crambe seed quality control programs. To evaluate vigour of crambe seed lots, the traditional accelerated ageing test should be executed for 48 hours at 41 °C and the SSAA method, for 24 hours at 41 or 45 °C. Seedling assessment should be carried out on the fourth day for both methodologies.
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