This article presents a test gas generation system designed to generate ppb level gas concentrations from gas cylinders. The focus is on permanent gases and volatile organic compounds (VOCs) for applications like indoor and outdoor air quality monitoring or breath analysis. In the design and the setup of the system, several issues regarding handling of trace gas concentrations have been considered, addressed and tested. This concerns not only the active fluidic components (flow controllers, valves), which have been chosen specifically for the task, but also the design of the fluidic tubing regarding dead volumes and delay times, which have been simulated for the chosen setup. Different tubing materials have been tested for their adsorption/desorption characteristics regarding naphthalene, a highly relevant gas for indoor air quality monitoring, which has generated high gas exchange times in a previous gas mixing system due to long time adsorption/desorption effects. Residual gas contaminations of the system and the selected carrier air supply have been detected and quantified using both an analytical method (GC-MS analysis according to ISO 16000-6) and a metal oxide semiconductor gas sensor, which detected a maximum contamination equivalent to 28 ppb of carbon monoxide. A measurement strategy for suppressing even this contamination has been devised, which allows the system to be used for gas sensor and gas sensor system characterization and calibration in the low ppb concentration range.

This paper presents on electromechanical characterization of thin film nickel-based wrinkled electrodes for dielectric elastomer (DE) applications. The investigation of a sandwich composed of a very soft and flexible elastomer carrying an ultrathin metallic electrode, together with its prestretch-dependent wrinkled structure of the electrode, facilitates the understanding of some of its interesting properties. Compared to conventional screen-printed carbon black electrodes, nickel-based thin film electrodes offer an ohmic resistance that is about 2 orders of magnitude lower. This remarkable feature makes it an advantageous electrode material alternative for the development of energy-efficient and high-frequency DE applications. Ultrathin (10−20 nm) layers are sputter deposited as electrodes onto either biaxially or, under pure-shear conditions, uniaxially prestretched silicone membranes. After the sputtering process, the membranes are allowed to relax whereby wrinkled out-of-plane buckled surfaces are obtained. With an initial resistance smaller than 400 Ω/square and a strong adhesion to the silicone, some electrode configurations are able to withstand strains up to 200% while remaining electrically conductive. A linear dependence of the capacitance on strain is revealed, as well as a long-term stability over 10 million cycles of mechanical stretching. All investigated thin film configurations of nickel and nickel−carbon films are suitable as compliant electrodes for DE actuators, as demonstrated by measuring the force characteristics with and without a high voltage. An increased level of prestretch shifts the resistance threshold of the electrode layers to even higher strain levels. In general, the best performance is achieved with pure metallic electrodes deposited on biaxially prestretched silicone membranes.

The availability of compliant actuators is essential for the development of soft robotic systems. Dielectric elastomers (DEs) represent a class of smart actuators which has gained a significant popularity in soft robotics, due to their unique mix of large deformation (>100%), lightweight, fast response, and low cost. A DE consists of a thin elastomer membrane coated with flexible electrodes on both sides. When a high voltage is applied to the electrodes, the membrane undergoes a controllable mechanical deformation. In order to produce a significant actuation stroke, a DE membrane must be coupled with a mechanical biasing system. Commonly used spring-like bias elements, however, are generally made of rigid materials such as steel, and thus they do not meet the compliance requirements of soft robotic applications. To overcome this issue, in this paper we propose a novel type of compliant mechanism as biasing elements for DE actuators, namely a three-dimensional polymeric dome. When properly designed, such types of mechanisms exhibit a region of negative stiffness in their force-displacement behavior. This feature, in combination with the intrinsic softness of the polymeric material, ensures large actuation strokes as well as compliance compatibility with soft robots. After presenting the novel biasing concept, the overall soft actuator design, manufacturing, and assembly are discussed. Finally, experimental characterization is conducted, and the suitability for soft robotic applications is assessed.

Due to their unique combination of features such as large deformation, high compliance, lightweight, energy efficiency, and scalability, dielectric elastomer (DE) transducers appear as highly promising for many application fields, such as soft robotics, wearables, as well as micro electro-mechanical systems (MEMS). To generate a stroke, a membrane DE actuator (DEA) must be coupled with a mechanical biasing system. It is well known that nonlinear elements, such as negative-rate biasing springs (NBS), permit a remarkable increase in the DEA stroke in comparison to standard linear springs. Common types of NBS, however, are generally manufactured with rigid components (e.g., steel beams, permanent magnets), thus they appear as unsuitable for the development of compliant actuators for soft robots and wearables. At the same time, rigid NBSs are hard to miniaturize and integrate in DE-based MEMS devices. This work presents a novel type of soft DEA system, in which a large stroke is obtained by using a fully polymeric dome as the NBS element. More specifically, in this paper we propose a model-based design procedure for high-performance DEAs, in which the stroke is maximized by properly optimizing the geometry of the biasing dome. First, a finite element model of the biasing system is introduced, describing how the geometric parameters of the dome affect its mechanical response. After conducting experimental calibration and validation, the model is used to develop a numerical design algorithm which finds the optimal dome geometry for a given DE membrane characteristics. Based on the optimized dome design, a soft DEA prototype is finally assembled and experimentally tested.

of new genotyping technologies requires awareness of their cerning utility for pharmacogenomics (PGx). In this review, s that can be applied in PGx research and clinical practice. ide variant (SNV) panels which contain a pre-selected panel short turnaround time and straightforward interpretation, ce. However, they are limited in their ability to assess rare sequencing (NGS) and long-read sequencing are promising h. Both NGS and long-read sequencing often provide more eciphering structural and rare variants compared to SNV mber of variants that can be identified, as well as the option hile useful for research, not all sequencing data can be ly, selecting the right technology is not a matter of fact but for the right problem. ype; phenotype; next generation sequencing; long-read (PGx) is developing rapidly. The first PGx dose psychiatric drugs were published in 2001, even before the . An increase in available evidence and the ambition to to the need for more comprehensive dosing guidelines and Pharmacogenetics Working Group (DPWG) was formed to 2]. In 2011, the Clinical Pharmacogenomics Implementation rrently, CPIC and the DPWG combined have issued PGx an 50 drugs and 21 genes (Table 1) [4,5].