High-quality tall mast and wind lidar measurements over the North and Baltic Seas are used to validate the wind climatology produced from winds simulated by the Weather, Research and Forecasting (WRF) model in analysis mode. Biases in annual mean wind speed between model and observations at heights around 100 m are smaller than 3.2% at offshore sites, except for those that are affected by the wake of a wind farm or the coastline. These biases are smaller than those obtained by using winds directly from the reanalysis. We study the sensitivity of the WRF-simulated wind climatology to various model setup parameters. The results of the year-long sensitivity simulations show that the long-term mean wind speed simulated by the WRF model offshore in the region studied is quite insensitive to the global reanalysis, the number of vertical levels, and the horizontal resolution of the sea surface temperature used as lower boundary conditions. Also, the strength and form (grid vs spectral) of the nudging is quite irrelevant for the mean wind speed at 100 m. Large sensitivity is found to the choice of boundary layer parametrization, and to the length of the period that is discarded as spin-up to produce a wind climatology. It is found that the spin-up period for the boundary layer winds is likely larger than 12 h over land and could affect the wind climatology for points offshore for quite a distance downstream from the coast.

Since the outbreak of the COVID-19 pandemic, singing activities for children and young people have been strictly regulated with far-reaching consequences for music education in schools and ensemble and choir singing in some places. This is also due to the fact, that there has been no reliable data available on aerosol emissions from adolescents speaking, singing, and shouting. By utilizing a laser particle counter in cleanroom conditions we show, that adolescents emit fewer aerosol particles during singing than what has been known so far for adults. In our data, the emission rates ranged from 16 P/s to 267 P/s for speaking, 141 P/s to 1240 P/s for singing, and 683 P/s to 4332 P/s for shouting. The data advocate an adaptation of existing risk management strategies and rules of conduct for groups of singing adolescents, like gatherings in an educational context, e.g. singing lessons or choir rehearsals.

In this study, emission rates of aerosols emitted by professional singers were measured with a laser particle counter under cleanroom conditions. The emission rates during singing varied between 753.4 and 6095.37~P/s. Emission rates for singing were compared with data for breathing and speaking. Significantly higher emission rates were found for singing. The growth rates between singing and speaking were between 3.97 and 99.54. Further, effects of vocal loudness and gender were investigated. The present study should support the efforts to improve the risk management in cases of possible aerogenic virus transmission, especially for choir singing.

In this study, emission rates of aerosols emitted by professional singers were measured with a laser particle counter under cleanroom conditions. The emission rates during singing varied between 753 and 6093 particles/sec with a median of 1537 particles/sec. Emission rates for singing were compared with data for breathing and speaking. Significantly higher emission rates were found for singing. The emission enhancements between singing and speaking were between 4.0 and 99.5 with a median of 17.4, largely due to higher sound pressure levels when singing. Further, significant effects of vocal loudness were found, whereas there were no significant differences between the investigated voice classifications. The present study supports the efforts to improve the risk management in cases of possible aerogenic virus transmission, especially for choir singing.

The cost of energy produced by onshore wind turbines is among the lowest available; however, onshore wind turbines are often positioned in a complex terrain, where the wind resources and wind conditions are quite uncertain due to the surrounding topography and/or vegetation. In this study, we use a scale model in a three-dimensional wind-testing chamber to show how minor changes in the terrain can result in significant differences in the flow at turbine height. These differences affect not only the power performance but also the life-time and maintenance costs of wind turbines, and hence, the economy and feasibility of wind turbine projects. We find that the mean wind, wind shear and turbulence level are extremely sensitive to the exact details of the terrain: a small modification of the edge of our scale model, results in a reduction of the estimated annual energy production by at least 50% and an increase in the turbulence level by a factor of five in the worst-case scenario with the most unfavorable wind direction. Wind farm developers should be aware that near escarpments destructive flows can occur and their extent is uncertain thus warranting on-site field measurements.

No abstract

No abstract

Carboxylesterases constitute a large enzyme family in insects, which is involved in diverse functions such as xenobiotic detoxification, lipid metabolism and reproduction. Phylogenetically, many insect carboxylesterases are represented by multienzyme clades, which are encoded by evolutionarily ancient gene clusters such as the α-Esterase cluster. Much in contrast to the vital importance attributed to carboxylesterases in general, the in vivo function of individual α-Esterase genes is largely unknown. This study employs a functional proteomics approach to identify esterolytic enzymes of the vinegar fly Drosophila melanogaster fat body. One of the fat body carboxylesterases, α-Esterase-7, was selected for mutational analysis by gene targeting to generate a deletion mutant fly. Phenotypic characterization of α-Esterase-7 null mutants and transgenic flies, which overexpress a chimeric α-Esterase-7:EGFP gene, reveals important functions of α-Esterase-7 in insecticide tolerance, lipid metabolism and lifespan control. The presented first deletion mutant of any α-Esterase in the model insect D. melanogaster generated by gene targeting not only provides experimental evidence for the endogenous functions of this gene family. It also offers an entry point for in vivo structure-function analyses of α-Esterase-7, which is of central importance for naturally occurring insecticide resistance in wild populations of various dipteran insect species.