Detection of the ATP-Dependent Nonmitochondrial Calcium Store in a Cell Surface-Derived Vesicle Fraction from Isolated Rat Hepatocytes

Lange, Julia; Schlieps, Karsten; Lange, Klaus; Brandt, Ursula; Knoll-Köhler, E

doi:10.1006/excr.1996.0316

Cited by 14 publications

(38 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent work on cellular regulation via specialized cell surface organelles, called microvilli, has yielded evidence for a common mechanism regulating various forms of transport and uptake processes such as glucose transport and ion #uxes via anion and cation channels (Lange, 1999;Lange et al, 1989Lange et al, , 1990Lange et al, , 1997Lange et al, , 1998Lange & Brandt, 1990a, b, 1993aGartzke & Lange, 1996, Gartzke et al, 1997. This type of regulation of metabolism and cell functions appears to be characteristic for di!erentiated cells.…”

Section: Special Surface Organization Of Differentiated Cells: Microvmentioning

confidence: 95%

“…This type of interaction of xenobiotics,including thapsigargin (Lange & Brandt, 1993a;Lange et al, 1997;Gartzke et al, 1997) and phorbol esters (data not published), with the cell surface is characterized by typical shape changes of microvilli indicative for an activated Ca> in#ux pathway by impairment of the cytoskeleton}membrane interaction (Gartzke & Lange, 1996;Gartzke et al, 1997). E Subtle mechanical load such as small bending of hair cell microvilli induced by acoustic waves or by streaming blood on microvilli of vascular endothelial cell activates the microvillar ion conduction pathway.…”

Section: Activation Of Ion -Uxes Through Microvillimentioning

confidence: 97%

“…The tip compartments of these organelles form a multitude of small pericellular spaces on the cell surface, termed entrance compartment, into which hexoses (Lange et al, 1989Lange & Brandt, 1990a, b) and ions (Lange & Brandt, 1993a, b;Lange et al, , 1997 can be taken up without restriction. The entrance compartment is, however, separated from the cytoplasm by a tightly aligned bundle of actin "laments representing an e!ective di!usion barrier (Fig.…”

Section: Special Surface Organization Of Differentiated Cells: Microvmentioning

confidence: 99%

“…A recently proposed mechanism of cellular Ca> signaling (reviewed in Lange, 1999), essentially depends on the dual function of the microvillar F-actin system acting both as a di!usion barrier and as a high-a$nity Ca> store (Lange, 1999;Lange & Brandt, 1993aLange et al, , 1997. Receptor-mediated F-actin dissociation/reorganization of the microvillar actin "laments at the same time releases Ca> from the F-actin store and opens the in#ux pathway for external Ca> into the cell.…”

Section: Activation Of Ion -Uxes Through Microvillimentioning

confidence: 99%

See 3 more Smart Citations

Microvillar Ion Channels: Cytoskeletal Modulation of Ion Fluxes

Lange¹

2000

Journal of Theoretical Biology

Self Cite

View full text Add to dashboard Cite

Section: Special Surface Organization Of Differentiated Cells: Microvmentioning

confidence: 95%

Section: Activation Of Ion -Uxes Through Microvillimentioning

confidence: 97%

Section: Special Surface Organization Of Differentiated Cells: Microvmentioning

confidence: 99%

Section: Activation Of Ion -Uxes Through Microvillimentioning

confidence: 99%

See 2 more Smart Citations

Microvillar Ion Channels: Cytoskeletal Modulation of Ion Fluxes

Lange¹

2000

Journal of Theoretical Biology

Self Cite

View full text Add to dashboard Cite

“…Similarly, in various other cell types mechanical strain induced ATP release was independent of CFTR [86,152]. The swelling-induced ATP release might occur via DIDS and/or NPPB inhibitable anion channels [30,[153][154][155][156] or by alternative pathways, propably by exocytosis. In human intestine 407 cells, hypoosmotic stress-induced release of ATP is inhibited by low intracellular Ca 2+ concentrations and by cytochalasin B, whereas the swelling-induced anion efflux is potentiated.…”

Section: Autocrine Regulation Of Rvd By Hormones and Transmittersmentioning

confidence: 99%

Mechanisms Sensing and Modulating Signals Arising From Cell Swelling

Jakab

Fuerst

Gschwentner

et al. 2002

Cell Physiol Biochem

125

100

View full text Add to dashboard Cite

Cell volume alterations are involved in numerous cellular events like epithelial transport, metabolic processes, hormone secretion, cell migration, proliferation and apoptosis. Above all it is a need for every cell to counteract osmotic cell swelling in order to avoid cell damage. The defence against excess cell swelling is accomplished by a reduction of the intracellular osmolarity by release of organic- or inorganic osmolytes from the cell or by synthesis of osmotically less active macromolecules from their specific subunits. De-spite the large amount of experimental data that has accumulated, the intracellular mechanisms underlying the sensing of cell volume perturbations and the activation of volume compensatory processes, commonly summarized as regulatory volume decrease (RVD), are still only partly revealed. Moving into this field opens a complex scenario of molecular rearrangements and interactions involving intracellular messengers such as calcium, phosphoinositides and inositolphosphates as well as phosphoryla-tion/dephosphorylation processes and cytoskeletal reorganization with marked cell type- and tissue specific variations. Even in one and the same cell type significant differences regarding the activated pathways during RVD may be evident. This makes it virtually im-possible to unambigously define common sensing- and sinaling pathways used by differ-ent cells to readjust their celll volume, even if all these pathways converge to the activa-tion of comparatively few sets of effectors serving for osmolyte extrusion, including ion channels and transporters. This review is aimed at providing an insight into the manifold cellular mechanisms and alterations occuring during cell swelling and RVD.

show abstract

Microvillar Ca++ signaling: A new view of an old problem

Lange¹

1999

J. Cell. Physiol.

Self Cite

View full text Add to dashboard Cite

Proceeding from the recent finding that the main components of the Ca++ signal pathway are located in small membrane protrusions on the surface of differentiated cells, called microvilli, a novel concept of cellular Ca++ signaling was developed. The main features of this concept can be summarized as follows: Microvilli are formed on the cell surface of differentiating or resting cells from exocytic membrane domains, growing out from the cell surface by elongation of an internal bundle of actin filaments. The microvillar tip membranes contain all functional important proteins synthesized such as ion channels and transporters for energy-providing substrates and structural components, which are, in rapidly growing undifferentiated cells, distributed over the whole cell surface by lateral diffusion. The microvillar shaft structure, a bundle of actin filaments, forms a dense cytoskeletal matrix tightly covered by the microvillar lipid membrane and represents an effective diffusion barrier separating the microvillar tip compartment (entrance compartment) from the cytoplasm. This diffusion barrier prevents the passage of low molecular components such as Ca++ glucose and other relevant substrates from the entrance compartment into the cytoplasm. The effectiveness of the actin-based diffusion barrier is modulated by various signal pathways and effectors, most importantly, by the actin-depolymerizing/reorganizing activity of the phospholipase C (PLC)-coupled Ca++ signaling. Moreover, the microvillar bundle of actin filaments plays a dual role in Ca++ signaling. It combines the function of a diffusion barrier, preventing Ca++ influx into the resting cell, with that of a high-affinity, ATP-dependent, and IP3-sensitive Ca++ store. Activation of Ca++ signaling via PLC-coupled receptors simultaneously empties Ca++ stores and activates the influx of external Ca++. The presented concept of Ca++ signaling is compatible with all established data on Ca++ signaling. Properties of Ca++ signaling, that could not be reconciled with the basic principles of the current hypothesis, are intrinsic properties of the new concept. Quantal Ca++ release, Ca(++)-induced Ca++ release (CICR), the coupling phenomen between the filling state of the Ca++ store and the activity of the Ca++ influx pathway, as well as the various yet unexplained complex kinetics of Ca++ uptake and release can be explained on a common mechanistic basis.

show abstract

Detection of the ATP-Dependent Nonmitochondrial Calcium Store in a Cell Surface-Derived Vesicle Fraction from Isolated Rat Hepatocytes

Cited by 14 publications

References 0 publications

Microvillar Ion Channels: Cytoskeletal Modulation of Ion Fluxes

Microvillar Ion Channels: Cytoskeletal Modulation of Ion Fluxes

Mechanisms Sensing and Modulating Signals Arising From Cell Swelling

Microvillar Ca++ signaling: A new view of an old problem

Contact Info

Product

Resources

About