Seafarers' jobs and living onboard as well as their mental health have been affected by the pandemic. These are indeed characteristics that may have a positive impact on seafarers, preventing them from quitting their jobs onboard. But, it is believed that on the other hand, there may also be influencing factors that make other seafarers remain still and firm in the performance of their work. The study aimed to determine attitude and cultural adaptation influencing the seafarers' work motivation of the seafarers in cruise line vessel such as attitude towards work environment as to the physical setting, emotional setting and social setting, culture adaptation in terms of communication skills and interpersonal relationship and work motivation in terms of the reward system and welfare and opportunity. Correlational design was applied to determine the relationship between the respondents' demographic characteristics and their attitude and culture adaptation and if attitude and culture adaptation influence their motivation to stay on their work. The participants in the study were 385 seafarers who work in the sections on food and beverage, housekeeping, hotel administration, and deck and engine department. Most of the respondents are 26 -41 years old and married, male-dominated, and working onboard for more than 10 years. They often observed work attitudes towards work environment, cultural adaptation, through communication skills and interpersonal relationships, and they are often motivated by reward system and welfare and opportunity. It is implied that competitive salaries, a comfortable workplace environment, a reward system, and welfare and opportunity for career progression may motivate the seafarers to stay on their job. Also, training on crisis management may help prepare future seafarers to be ready to adapt to life onboard ships.