Mexico serves as a global model for advances in rabies prevention and control in dogs. The Mexican Ministry of Health (MMH) annual application of approximately 16 million doses of parenteral rabies vaccine has resulted in significant reductions in canine rabies during the past 20 years. One collateral parameter of rabies programs is dog population management. Enhanced public awareness is critical to reinforce responsible pet ownership. Surgical spaying and neutering remain important to prevent reproduction, but are impractical for achieving dog population management goals. GonaCon™, an anti-gonadotropin releasing hormone (GnRH) vaccine, was initially tested in captive female dogs on the Navajo Nation, 2008. The MMH led this international collaborative study on an improved formulation of GonaCon™ in captive dogs with local representatives in Hidalgo, Mexico in 2011. This study contained 20 bitches assigned to Group A (6 control), Group B (7 GonaCon™), and Group C (7 GonaCon™ and rabies vaccine). Vaccines were delivered IM. Animals were placed under observation and evaluated during the 61-day trial. Clinically, all dogs behaved normally. No limping or prostration was observed, in spite of minor muscle atrophy post-mortem in the left hind leg of dogs that received GonaCon™. Two dogs that began the study pregnant give birth to healthy pups. Dogs that received a GonaCon™ injection had macro and microscopic lesions consistent with prior findings, but the adverse injection effects were less frequent and lower in intensity. Both vaccines were immunogenic based on significant increases in rabies virus neutralizing antibodies and anti-GnRH antibodies in treatment Groups B and C. Simultaneous administration of GonaCon™ and rabies vaccine in Group C did not affect immunogenicity. Progesterone was suppressed significantly in comparison to controls. Future studies that monitor fertility through multiple breeding cycles represent a research need to determine the value of integrating this vaccine into dog rabies management.
The objective of the present study was to determine the effect of the application of a nanoemulsion made of orange essential oil and Opuntia oligacantha extract on avocado quality during postharvest. The nanoemulsion was applied as a coating in whole fruits, and the following treatments were assessed: concentrated nanoemulsion (CN), 50% nanoemulsion (N50), 25% nanoemulsion (N25) and control (C). Weight loss, firmness, polyphenol oxidase (PPO) activity, total soluble solids, pH, external and internal colour, total phenols, total flavonoids, antioxidant activity by 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH), while the structural evaluation of the epicarp was assessed through histological cuts. Significant differences were found (p < 0.05) among the treatments in all the response variables. The best results were with the N50 and N25 treatments for firmness and weight loss, finding that the activity of the PPO was diminished, and a delay in the darkening was observed in the coated fruits. Furthermore, the nanoemulsion treatments maintained the total phenol and total flavonoid contents and potentiated antioxidant activity at 60 days. This histological study showed that the nanoemulsion has a delaying effect on the maturation of the epicarp. The results indicate that using this nanoemulsion as a coating is an effective alternative to improve the postharvest life of avocado.
Los microorganismos causantes de enfermedades en el humano y animales están en constante evolución, por lo que es de vital importancia encontrar alternativas para su tratamiento y prevención. La resistencia antimicrobiana, definida por la OMS como la capacidad que tienen los microorganismos de impedir que los antimicrobianos actúen contra ellos, es un problema creciente en nuestra sociedad. En el año 2016, la Organización Mundial de la Salud publicó un informe en el que se estimó que para ese año se atribuían 700 000 muertes a la resistencia a antimicrobianos en el mundo, y que la cifra puede ascender hasta 10 millones de muertes en los próximos 35 años si no se toman medidas adecuadas. Para el siguiente año, la OMS publicó un reporte donde se mencionan 51 nuevos antimicrobianos que pueden ser una alternativa al tratamiento de algunas enfermedades, sin embargo, solo 8 de estas moléculas se consideran con potencial terapéutico. El uso de insectos en la medicina no es considerada una práctica reciente, ya que hay referencias de ella desde las primeras civilizaciones y ha sido demostrado que tiene aspectos benéficos no solo para la nutrición, sino para ámbitos de salud. Por ejemplo, los péptidos antimicrobianos se han descrito en muchas especies de organismos: hongos, plantas, humanos e insectos, siendo estos últimos en los que más se han descrito péptidos antimicrobianos eficaces.
El género Tithonia, ha sido ampliamente estudiado para determinar sus efectos bioactivos de interés farmacéutico y nutricional, por lo que el objetivo del presente estudio fue determinar el efecto antioxidante y antibacterial de 5 extractos de Tithonia tubaeformis, obtenidos con solventes de polaridad ascendente, hexano, diclorometano, acetato de etilo, metanol y etanol. Se identificó la presencia de taninos, alcaloides y fenoles totales. Se midió el efecto antioxidante a los radicales ABTS, DPPH y poder reductor al ion férrico, se determinó la CMI de cada extracto sobre Escherichia. coli, Salmonella typhimurium, Salmonella enteritidis y Listeria monocytogenes. Los resultados obtenidos mostraron que los cinco extractos tienen efecto antioxidante sobre los radicales ABTS, DPPH, y potencial reductor al ion férrico; sin embargo, los extractos que presentaron un efecto estadísticamente significativo (P<0.05) fueron metanol y etanol. Los extractos de metanol, etanol y acetato de etilo mostraron actividad antimicrobiana, siendo el solvente de etanol el que mayor actividad antibacterial tuvo, con una CMI de 1 mg/mLsobre Escherichia coli y 3 mg/mL sobre Escherichia. coli, Salmonella typhimurium, Salmonella enteritidis y Listeria monocytogenes. Estos resultados sugieren que Tithonia tubaeformis puede ser de interés farmacéutico y nutricional por los efectos bioactivos de los fitoquímicos presentes en sus extractos.
This study was aimed at evaluating the effect of a nanoemulsion containing the bioactive compounds of orange essential oil and xoconostle (Opuntia oligacantha C.F. Först) on maintaining and improving the quality of the shelf life of tomato fruits. The nanoemulsion was applied as a coating on the whole fruits during physiological maturity; the treatments were thus: Control 1 without coating (C1); Control 2 with food-grade mineral oil coating (C2); and nanoemulsions that were diluted with mineral oil at 2.5% (DN2.5), 5% (DN5), 10% (DN10), and 20% (DN20). Further, the following parameters were determined for 21 days: the percentage weight loss, firmness, colour, pH, titratable acidity, total soluble solids, ascorbic acid content, total phenols, flavonoids, tannins, antioxidant activities DPPH and ABTS, and the histological evaluation of the pericarp of the fruits. Significant differences (p < 0.05) were observed during the treatments; DN10 and DN20 obtained the best weight loss results (3.27 ± 0.31% and 3.71 ± 0.30%, respectively) compared with C1 and C2. The DN5 and DN20 textures exhibited the highest firmness (11.56 ± 0.33 and 11.89 ± 1.04 N, respectively). The antioxidant activity (DPPH on Day 21) was higher in the DN20 treatment (48.19 ± 0.95%) compared with in C1 (39.52 ± 0.30%) and C2 (38.14 ± 0.76%). Histological evaluation revealed that the nanoemulsion coating allowed a slower maturation of the cells in the pericarp of the fruits. The nanoemulsion, as a coat, improved the quality and valuable life of the tomato regarding its physicochemical and antioxidant properties, thus availing an effective alternative for conserving this fruit.
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