Resumo -O objetivo deste trabalho foi selecionar substratos favoráveis à associação micorrízica na produção de mudas micropropagadas de porta-enxertos de videira. Os porta-enxertos SO4 e Paulsen 1103 foram aclimatizados por três semanas em bandejas alveoladas, contendo seis substratos (à base de solo, composto termofílico, casca de arroz carbonizada, vermiculita, areia e um substrato comercial), com e sem inoculação micorrízica. No final da aclimatização e dez semanas depois, avaliaram-se a colonização micorrízica, a matéria seca de parte aérea e o comprimento radicular das plantas. A intensidade de colonização micorrízica variou em razão dos porta-enxertos e dos substratos utilizados na aclimatização, havendo interação significativa entre os dois fatores. O substrato comercial proporcionou a maior produção de biomassa, mas promoveu a menor colonização micorrízica em todos os porta-enxertos. As combinações mais favoráveis de produção de biomassa vegetal e de taxa de colonização micorrízica ocorreram no porta-enxerto Paulsen 1103, no substrato à base de solo, composto termofílico e areia e, no porta-enxerto SO4, no substrato à base de solo, composto termofílico e vermiculita.Termos para indexação: Vitis, micorriza, muda, micropropagação, biomassa. Evaluation of substrates for mycorrhization and weaning of two micropropagated grapevine rootstocksAbstract -The aim of this work was to select substrates conductive to the establishment of arbuscular mycorrhizas in the production of micropropagated grapevine rootstocks. Micropropagated SO4 and Paulsen 1103 rootstock plantlets were weaned for three weeks in trays with mycorrhizal fungalinoculated or uninoculated substrates (prepared with soil, compost, calcined rice hulls, vermiculite and sand, besides a commercial substrate). At the end of the weaning period and ten weeks after, shoot and root growth and mycorrhizal root colonization were evaluated. Mycorrhizal root colonization varied according to the rootstock and substrates, with a significant interaction between these factors. The commercial substrate was associated with the highest increase in shoot and root mass, but it decreased mycorrhizal colonization. The best substrate for Paulsen 1103 rootstock growth and mycorrhizal inoculation was prepared with soil, compost and sand and, for the SO4 rootstock the substrate prepared with soil, compost and vermiculite.
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