Bioemulsifier is bioactive molecules produced by different microorganisms with reducing power and surface and interfacial tension. Among the microorganisms producing this molecule is yeast, which can produce different bioemulsifiers in different substrates. Undoubtedly, this biomolecule has excellent potential for industrial applications, but high production costs are the biggest problem in production. Aiming at cost reduction the present study using crude residual glycerol for biosurfactant production by Yarrowia lipolytica. Then isolates were grown in residual glycerol compound medium, rotating 200 rpm at 28ºC for 48 hours. Bioemulsifier production was observed by analysis of dry biomass, pH, surface tension and emulsification index. The results indicated that the emulsion produced from biosurfactant using glycerol as a carbon source by Y. lipolytica has the potential for bioemulsifier production. All isolates obtained similar results for all analyzes, indicating that this species has a linear production among the isolates. Biomass reached 10.08 ± 0.62 g.L-1 , there was a sharp drop in pH reaching 4.6, surface tension averaged 41.7 mN.m-1 and emulsification index reached 56%. The isolates tested show potential for bioemulsifier production using glycerol as an unconventional carbon source.
A B S T R A C TThe development of research for the production of biofuels using low-cost substrate has become more relevant in recent years. These include reuse of residues such as crude residual glycerol from biodiesel (CRG) and cheese whey (CW) from the dairy industry. This study evaluated the ethanol production by isolates of the yeast Kluyveromyces marxianus using agroindustrial residues as an alternative source of carbon. The cultures were rotated 100 rpm at 30°C for 24 h. The ethanol production was observed in both media; however, in the CW higher values of ethanol was observed about the CRG. The results showed that K. marxianus isolates were adapted to the use of lactose present in cheese whey as a source of carbon for the production of ethanol with concentrations ranging from 11.41 to 19.9 g.L -1 , but did not demonstrate efficiency in the use of crude glycerol for this purpose.
RESUMOA substituição de nematicidas químicos por produtos ecologicamente sustentáveis é uma importante ferramenta no gerenciamento microbiológico do solo e no manejo de fitonematoides. O objetivo do presente trabalho foi avaliar o potencial de isolados fúngicos na redução do parasitismo de Meloidogyne incognita em plantas de tomateiro. O experimento foi conduzido no Centro de Tecnologias Estratégicas do Nordeste. Em ambiente protegido foram testados três isolados fúngicos e dois produtos comerciais. Ao 15º dia do início da germinação, procedeu-se com a inoculação dos substratos com os controles (isolados fúngicos do gênero Trichoderma e Gongronella ou produto comercial) e após cinco dias da inoculação dos substratos, procedeu-se a inoculação de 1.100 ovos de M. incognita por planta, exceto a testemunha geral. Aos 45 dias após a inoculação, foram avaliadas a biomassa fresca da parte aérea e raiz, número de galhas no sistema radicular (NGSR), número de ovos (NO), fator de reprodução (FR) e pigmentos fotossintetizantes. Para os teores de todos os pigmentos avaliados, o produto 1 e o isolado CTFN-18 apresentaram médias inferiores às obtidas pela testemunha. Apesar de alguns tratamentos terem influência negativa no teor de pigmentos, não foram observadas alterações na biomassa fresca da parte aérea e raiz. Para o NGSR apenas o produto 1 diferiu dos demais tratamentos, apresentando média superior. Tanto para o NO quanto para o FR, o isolado CTFN-18 não diferiu significativamente do produto 2 que apresentou as menores médias, o que aponta o grande potencial do isolado CTFN-18 para o controle biológico de M. incognita.Palavras-chave: biocontrole, nematoides das galhas, trichoderma.
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