This study was conducted in order to evaluate the transmission of caprine lentivirus to sheep using different experimental groups. The first one (colostrum group) was formed by nine lambs receiving colostrum from goats positive for small ruminant lentiviruses (SRLV). The second group (milk group) was established by nine lambs that received milk of these goats. Third was a control group, consisting of lambs that suckled colostrum and milk of negative mothers. Another experimental group (contact group) was formed by eight adult sheep, confined with two naturally infected goats. The groups were monitored by immunoblotting (IB), enzyme-linked immunosorbent assay (ELISA), agar gel immunodiffusion (AGID) and nested polymerase chain reaction (nPCR). All lambs that suckled colostrum and milk of infected goats and six sheep of the contact group had positive results in the nPCR, although seroconversion was detected only in three of the exposed animals, with no clinical lentiviruses manifestation, in 720 days of observation. There was a close relationship between viral sequences obtained from infected animals and the prototype CAEV-Cork. Thus, it was concluded that SRLV can be transmitted from goats to sheep, however, the degree of adaptation of the virus strain to the host species probably interferes with the infection persistence and seroconversion rate.
RESUMOCom o objetivo de analisar a ocorrência de anticorpos contra Brucella ovis em ovinos no semiárido baiano, este inquérito foi conduzido em rebanhos da microrregião de Juazeiro, Bahia. O teste de imunodifusão em gel de ágar (IDGA) foi utilizado para examinar 694 amostras de soro de 58 rebanhos. Anticorpos para B. ovis foram observados em cinco (0,72%) dos animais investigados. Acredita-se que o baixo número de soropositivos deva-se às características dos sistemas de produção, com predominância do tipo de exploração extensiva de animais localmente adaptados, mestiços e sem raça definida para a produção de carne e pele, com baixa produtividade e tecnificação.PALAVRAS-CHAVE: Brucelose ovina, epidemiologia, IDGA, ocorrência. ABSTRACT SERO-EPIDEMIOLOGICAL SURVEY FOR BRUCELLA OVIS INFECTION IN SHEEP FLOCKS OF BAHIA STATE, BRAZIL. In order to analyze the occurrence of antibodies toBrucella ovis in sheep in a semi-arid region, this survey was conducted in the micro-region of Juazeiro, Bahia State, Brazil. The agar gel immunodifusion test (AGID) was used to examine 694 serum samples of 58 flocks. Antibodies to B. ovis were found in 5 (0.72%) of the investigated animals. It is believed that this low number of positive cases is related to the characteristics of the production. The predominant system is the extensive one, with presence of locally adapted breeds and crossbred animals, aiming at the production of meat and skin, with low productivity rates and technical development.
A introdução de novas raças, com o objetivo de aumentar a produtividade, levou à alteração do perfil sanitário dos rebanhos. Dentre as enfermidades introduzidas no Brasil, destacam-se as lentiviroses de pequenos ruminantes (LVPR), representadas pela artrite-encefalite caprina (CAE) e pela maedi-visna (MV). Devido à importância econômica da ovinocultura para a microrregião de Juazeiro-Bahia e à escassez de dados sobre a lentivirose em ovinos, buscou-se obter a prevalência da MV. Foram avaliados 919 soros por imunodifusão em gel de ágar (IDGA). As amostras foram colhidas nos oito municípios que compõem essa microrregião (Juazeiro,
Este trabalho teve como finalidade verificar a prevalência sorológica da lentivirose caprina (LVC) na microrregião de Juazeiro, na Bahia, por meio da técnica de imunodifusão em gel de agar (IDGA), bem como caracterizar os sistemas de criação da região. Para tal, foram avaliadas 693 amostras de soros sanguíneos de caprinos de 46 propriedades rurais em diferentes localidades, pertencentes aos 8 municípios formadores da microrregião (Campo Alegre de Lourdes, Casa Nova, Curaçá, Juazeiro, Pilão Arcado, Remanso, Sento Sé e Sobradinho). Na realização das visitas, aplicou-se um questionário com ênfase nas informações referentes ao manejo sanitário. Das propriedades visitadas, todas apresentavam sistema de criação extensivo, com predomínio de animais sem raça definida, baixa produtividade e baixo índice de tecnificação, visando principalmente à obtenção de carne. As principais enfermidades relatadas foram linfadenite caseosa, diarreias, ectoparasitoses e ceratoconjuntivite. Quanto à soroprevalência, 0,29% (2/693) das amostras apresentaram sorologia positiva para a LVC. Os animais positivos pertenciam à mesma propriedade, no município de Curaçá, que apresentou 12,5% (1/8) de propriedades positivas, contrastando com 2,17% (1/46) de soroprevalência total dos rebanhos visitados. Estes resultados sugerem, portanto, a necessidade da efetivação de medidas preventivas na região, principalmente no momento em que se verifica a importação de animais para melhoramento genético.
Pesq. Vet. Bras. 34(2):103-108, fevereiro 2014 103 RESUMO.-O objetivo do presente trabalho foi utilizar mé-todos bacteriológicos e moleculares para a identificação do Mycobacterium bovis em lesões observadas em carcaças de bovinos durante a inspeção post mortem de rotina em matadouros-frigoríficos com serviço de inspeção oficial. Foi acompanhado o abate e a inspeção de 825.394 bovinos, sadios ao exame ante mortem pelo serviço de inspeção oficial em dez matadouros-frigoríficos do estado da Bahia. Carcaça de 180 bovinos apresentaram lesões sugestivas de tuberculose e por outras linfadenites. No isolamento bacteriano, 25 amostras apresentaram crescimento disgônico de colônias de coloração creme-amareladas em meio de cultura Stonebrink-Leslie. Desses isolados, 14 foram identifi- Múltiplas estirpes de isolados de Mycobacterium bovisidentificados por tipagem molecular em bovinos abatidos em matadouros-frigoríficos The aim of this study was to use bacteriological and molecular methods to identify Mycobacterium bovis in lesions observed in cattle carcasses during routine post-mortem inspection in slaughterhouses with official inspection service. It was accompanied the slaughter and inspection of 825,394 cattle, healthy ante mortem examination by the official inspection service in ten slaughterhouses in the state of Bahia. Carcasses of 180 cattle presented lesions suggestive of tuberculosis and other lymphadenitis. In bacterial isolation, 25 samples showed dysgonic growth of colonies of creamy-yellow in medium-Stonebrink Leslie. From these isolates, 14 were identified as M. bovis and the multiplex PCR technique spoligotyping was discriminated against eight different spoligotypes of M. bovis, seven previously described in the literature and a new spoligotypes without former description. The major spoligotypes was SB0121, with five samples which has been described in Brazil and other countries, followed by two clusters, SB295 and SB1055, with two isolates each. The SB1145 and SB1648 spoligotypes were reported only in Brazil and Denmark, respectively. The spoligotypes SB140 has been found in Brazil, Argentina, Uruguay and Paraguay. These results demonstrate that the spoligotypes obtained are shared, so far, among Brazilian states and among Latin America and Europe. Thus, molecular discrimination of isolates of M. bovis by Spoligotyping constitutes a tool for epidemiological studies of bovine tuberculosis in the state of Bahia. INDEX TERMS: Mycobacterium bovis, mycobacteria, molecular epidemiology, spoligotyping, tuberculosis, zoonosis, cattle. (Brasil 2006, Heinemann et al. 2008.A tuberculose no homem é causada principalmente pelo Mycobacterium tuberculosis, mas a doença causada por M. bovis tem sido reportada e representa um risco para a saúde pública (Parreiras et al. 2012, Reyes et al. 2012. Em 2011, 8,7 milhões de pessoas adoeceram e 1,4 milhões de óbitos anuais foi determinado pela tuberculose. A maioria dos óbitos ocorre em países de baixa e média renda e está entre as três principais causas de mortes em mulheres ent...
ABSTRACT.-Carvalho V.S., Araújo B.R., Vasconcelos T.C., Chiminazzo C., Costa Neto A.O., Ayres M.C.C., Guimarães J. In this paper, Santa Inês crossbred sheep with footrot were evaluated, checking the changes on leukogram and the acute phase proteins. In the ϐirst experiment, 70 females were divided into three groups according to their podal scores: a control group (G1) with score 0, scald group (G2) with score 1 or 2 and a footrot group (G3) with score 3, 4 or 5. During two months, the clinical course and its effect on leukogram were observed at ϐive moments: M1 (day 0), M2 (day 15), M3 (day 30), M4 (day 45) and M5 (day 60). The intensity of the white blood cell count was low magnitude, observing signiϐicant changes (p <0.05) as a mild leukocytosis (G3, M4), attributed to neutrophilia and a slight increase in the total number of monocytes (G3, M2 and M3) despite the extensive damage and tissue necrosis existed during the ϐinal stage of the disease. In the second experiment, 105 animals from seven farms were used with the objective of isolating the causative agent and to evaluate the effect of disease on total plasma protein and acute phase proteins. The animals were also divided into three groups: control, scald and footrot. In all farms, the isolation of Dichelobacter nodosus was regarded. For the different stages of the disease on the proteins studied there was no signiϐicant correlation (p<0.05) but the haptoglobin in scald and footrot groups showed higher average than the control group. Based on these results, we conclude that the acute phase proteins studied were not efϐicient in the characterization of the phases of the disease and the leukocyte response was mild, making it difϐicult to use for this purpose.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.