Retinoids have been shown to enhance developmental competence of the oocyte in cattle, sheep and pigs. In this study we investigated whether exogenous retinol stimulates the bovine oocyte during its intrafollicular growth and the time limits of exposure to exogenous retinol. In addition, we also determined the efficiency of ovum pick-up techniques in combination with retinol treatment and the viability of embryos after transfer to recipients. In Experiment 1, heifers were injected with retinol or vehicle, and concentrations of retinol in the blood were analysed on Day 0 (prior to injection), Day 1 and, together with follicular fluid, Day 4. Blood retinol increased by Day 1 and cleared on Day 4, but retinol remained higher within the follicle. In Experiment 2, oocyte donors were injected weekly with retinol or vehicle four times during a twice-per-week cycle of eight recovery sessions (starting 4 days before the first session), followed by a second eight-session cycle without treatment. Oocytes recovered were fertilized and culturedin vitro.Retinol treatment yielded higher numbers of low-quality oocytes throughout, although retinol measured during cycles did not change. Total oocytes, and morulae and blastocyst rates, increased during the first five sessions following treatment with retinol. As previously shown with oocytes from slaughterhouse ovaries, retinoic acid stimulated blastocyst development. Following transfer to recipients, blastocysts from oocytes exposed to retinol were unable to establish pregnancy. Our study confirms the existence of an effect of retinol on the intrafollicular oocyte in the cow and provides evidence regarding the teratogenic effect of retinol.
The Iberian hare (Lepus granatensis Rosenhauer, 1856) has the western distribution limit in the south of the dorsal galega. This work provides new data on the status of the species over 16 years of sampling. Indices of relative abundance of Iberian hare were determined in the dorsal galega through night censuses carried out in each season. During the years 2006-2020, a total of 2,500 km was gone down along forest tracks in the Suido and Faro de Avión mountain ranges, spread over 196 days. The relative abundance for the hare ranged between 0.07 and 0.30 ind/km for the Faro de Avión with an average of 0.18 ± 0.06 ind/km, and between 0.02 and 0.22 ind/km for the Suido, with an average of 0.07 ± 0.06 ind/km. The monitoring along the years of the studied population supports scientific decision-making for conservation and hunting management purposes.
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