Efficient one-step direct transfer to recipients of thawed bovine embryos cultured in vitro and frozen in chemically defined medium

Gómez, E.; Carrocera, S.; Martín, David; Pérez-Jánez, Juan José; Prendes, J.M.; Prendes, José Manuel; Vázquez, A; Murillo, Antonio; Gimeno, Isabel; Muñoz, M.

doi:10.1016/j.theriogenology.2020.01.056

Cited by 43 publications

(54 citation statements)

References 48 publications

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“…There are also good prospects that modifications of media for IVP can enhance cryosurvival of embryos. Recently, Gómez et al (2020) reported that IVP embryos cultured with BSA to day 6 and then without protein to day 7 achieved a pregnancy rate following freezing and direct transfer that was similar to that following transfer of fresh embryos ( Figure 6 ). Freezing was performed in ethylene glycol with a synthetic protein substitute called CRYO3.…”

Section: Some Ways Forwardmentioning

confidence: 68%

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The incompletely fulfilled promise of embryo transfer in cattle—why aren’t pregnancy rates greater and what can we do about it?

Hansen

2020

Journal of Animal Science

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Typically, bovine embryos are transferred into recipient females about day 7 after estrus or anticipated ovulation, when the embryo has reached the blastocyst stage of development. All the biological and technical causes for failure of a female to produce a blastocyst 7 d after natural or artificial insemination (AI) are avoided when a blastocyst-stage embryo is transferred into the female. It is reasonable to expect, therefore, that pregnancy success would be higher for embryo transfer (ET) recipients than for inseminated females. This expectation is not usually met unless the recipient is exposed to heat stress or is classified as a repeat-breeder female. Rather, pregnancy success is generally similar for ET and AI. The implication is that either one or more of the technical aspects of ET have not yet been optimized or that underlying female fertility that causes an embryo to die before day 7 also causes it to die later in pregnancy. Improvements in pregnancy success after ET will depend upon making a better embryo, improving uterine receptivity, and forging new tools for production and transfer of embryos. Key to accelerating progress in improving pregnancy rates will be the identification of phenotypes or phenomes that allow the prediction of embryo competence for survival and maternal capacity to support embryonic development.

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Section: Some Ways Forwardmentioning

confidence: 68%

“…Frozen embryos were thawed and then transferred directly without removing cryoprotectants. Results are from Gómez et al (2020) .…”

Section: Some Ways Forwardmentioning

confidence: 99%

The incompletely fulfilled promise of embryo transfer in cattle—why aren’t pregnancy rates greater and what can we do about it?

Hansen

2020

Journal of Animal Science

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“…The PR gap between in vivo fresh and frozen embryos is very small, but not so with in vitro‐derived embryos. Currently, the most popular cryopreservation method for IVP embryos is still vitrification (Vajta, 2000), although slow freezing is earning more followers (Bruyere et al, 2012; Gómez et al, 2020). Vitrification has based its strengths on an adequate supply of more suitable embryos to freeze and a lower cost of equipment (Vajta & Kuwayama, 2006) and may be more suitable for IVP higher lipid content embryos than the slow freezing technique; however, current warming procedures and carrier design are not compatible for DT.…”

Section: Ivp Embryo Cryotolerancementioning

confidence: 99%

“…Several strategies have been applied to reduce the chilling sensitivity of in vitro produced bovine embryos by adapting culture conditions to reduce the lipid content in the cytoplasm (Braga, Komninou, Vieira, & Mondadori, 2019). Serum reduction [≤5% v/v] (Holm, Booth, Schmidt, Greve, & Callesen, 1999; Sudano et al, 2011) or complete substitution has been a main target of such research (Gómez et al, 2020; Rizos et al, 2003). By keeping a sufficient concentration of serum and adding lipolytic substances, which restrict fatty acid synthesis, one may enhance embryo quality and viability after cryopreservation (Barcelo‐Fimbres & Seidel, 2007; Paschoal et al., 2014; Sudano et al, 2011).…”

Section: Ivp Embryo Cryotolerancementioning

confidence: 99%

“…Acceptable commercial pregnancy rates can be accomplished using BSA up to day 6 of the culture period with no protein source for an additional 24h before being subjected to the slow freezing procedure. This aforementioned methodology combined with protein‐free freezing media may favour embryo implantation performance, lower miscarriage rates and produce normal birth weights (Gómez et al, 2020). Previous studies using bovine oviduct epithelial cells revealed a high interaction between gametes during fertilization and during early embryo development (Rottmayer et al, 2006).…”

Section: Improving Ivp Embryo Cryotolerance Through In Vitro Culture mentioning

confidence: 99%

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Recent progress in bovine in vitro‐derived embryo cryotolerance: Impact of in vitro culture systems, advances in cryopreservation and future considerations

Ferré

Kjelland

Taiyeb³

et al. 2020

Reprod Domestic Animals

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Contents Cryopreservation of in vitro‐derived bovine embryos is a crucial step for the widespread reproduction and conservation of valuable high‐merit animals. Given the current popularity of bovine in vitro embryo production (IVP), there is a demand for a highly efficient ultra‐low temperature storage method in order to maximize donor ovum pickup (OPU) turn‐over, recipient availability/utilization and domestic/overseas commercial trading opportunities. However, IVP bovine embryos are still very sensitive to chilling and cryopreservation, and despite recent progress, a convenient (simple and robust) protocol has not yet been developed. At the moment, there are two methods for bovine IVP embryo cryopreservation: slow programmable freezing and vitrification. Both of the aforementioned techniques have pros and cons. While controlled‐rate slow cooling can easily be adapted for direct transfer (DT), ice crystal formation remains an issue. On the other hand, vitrification solved this problem but the possibility of successful DT commercial incorporation remains to be determined. Moreover, simplification of the vitrification protocol (including warming) through the use of an in‐straw dilution without the use of a microscope is a prerequisite for its use under farm conditions. This review summarizes the bovine IVP embryo cryopreservation achievements, strengths and limitations of both freezing systems and prospective improvements to enhance cryosurvival, as well as perspectives on future directions of this assisted reproductive technology.

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Abnormal Offspring Syndrome

Rivera¹,

Donnelly²,

Patel³

et al. 2021

Bovine Reproduction

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Efficient one-step direct transfer to recipients of thawed bovine embryos cultured in vitro and frozen in chemically defined medium

Cited by 43 publications

References 48 publications

The incompletely fulfilled promise of embryo transfer in cattle—why aren’t pregnancy rates greater and what can we do about it?

The incompletely fulfilled promise of embryo transfer in cattle—why aren’t pregnancy rates greater and what can we do about it?

Recent progress in bovine in vitro‐derived embryo cryotolerance: Impact of in vitro culture systems, advances in cryopreservation and future considerations

Abnormal Offspring Syndrome

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