With new digital systems, the role of the microscope as an irreplaceable instrument in the teaching of histology has been called into question. In this study academic performance and student perception for two learning methods were compared: digital and light microscopy, in the muscle tissue unit of the morphology course for first-year undergraduate dentistry at Universidad de los Andes, Santiago, Chile. 92 students were divided into 2 groups: Group 1: light microscopy and Group 2: digital microscope in a web application. All participants observed the same striate muscle, cardiac striated muscle, and smooth muscle samples. An academic performance test was applied (diagnostic and individual structures identify ability). A perception test was conducted after everyone had learned with both systems. For data analysis the T student test and central tendency with their standard deviation was used. In the cognitive evaluation, the median grades were 5.4 for group 1 and 5.7 for group 2. In the perception survey, 73.24 % considered the virtual microscope evaluation more fair. It was concluded that the use of virtual microscope tends to have better results than light microscopy. Further investigation is needed to evaluate this new teaching instrument.
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