To determine if the marine foods of animal origin that are sold in establishments in the port of Chabihau, Yucatan, Mexico, represent potential risk factors for the Vibrio alginolyticus species for the development of acute gastroenteritis, wound infection, ear infection and primary septicemia. Three hundred and ninety seafood samples were studied: [298 (76.41%) raw, 8 (2.05%) marinated without heat, 77 (19.74%) partially cooked with heat and 7 (1.79%) completely cooked with heat]. For the homogenization and enrichment of the samples, as well as for the isolation and identification of the Vibrio alginolyticus species, we proceeded according to the methodology described in the eighth edition of the Bacteriological Analytical Manual (FDA). The criteria used in conducting the hypothesis tests for the difference between two percentages was based on the recommendations made by Cochran. The prevalences obtained in raw seafood, marinated without heat, partially cooked with heat and completely cooked with heat were, respectively, 7.72% (23/298), 0.00% (0/8), 7.79% (6/77) and 0.00% (0/7). In 29 (7.44%) samples an equal number of strains were isolated whose biochemical characteristics corresponded to the Vibrio alginolyticus species. It is concluded that raw seafood and partially cooked seafood represent potential risk factors for the Vibrio alginolyticus species for the development of acute gastroenteritis, wound infection, ear infection and primary septicemia.
Background and objective. Koch et al., reported that the Waist/Hip Index more accurately predicts cardiovascular risk factors and mortality. Huaman et al., reported that the Waist/Height Index can be used as a diagnostic test for metabolic syndrome, since it has a high significant value. To determine whether two anthropometric indices have sufficient prognostic efficiency or moderate prognostic efficiency by combining sensitivity and specificity using positive and negative likelihood ratios in a single expression. Material and methods. Quantitative epistemological approach. Descriptive observational epidemiological study without directionality and with prospective temporality. Three husband adult patients of both genders who attended the Integral Hospital "Jose Maria Morelos" were studied. As a reference test or Gold Standard was used the Lorentz Equation as a reference test. Results. The results for the positive likelihood ratios corresponded to 13.41 and 1.72 for the anthropometric indices Waist/Height Index and Waist/Hip Index, respectively. The results for the negative likelihood ratios corresponded, respectively, to 0.07 and 0.48 for the anthropometric indices Waist/Height Index and Waist/Hip Index. Conclusions. The best anthropometric index for the diagnosis of overweight and obesity pathological nutritional situations corresponds to the Waist/Height Index, since the results of the positive and negative likelihood ratios report sufficient prognostic efficiencies. On the other hand, the results of the positive and negative likelihood ratios report, respectively, negligible prognostic efficiency and poor prognostic efficiency for the Waist/Hip Index.
Low birth weight is an indicator that allows predicting the probability of survival of a child. In fact, there is an exponential relationship between weight deficit, gestational age, and perinatal mortality. In addition, it is important to indicate that a percentage of term children (37 ≤ weeks of gestation ≤ 41) who have low birth weight present with various sequelae of variable severity –especially in the neurological sphere– and hence the importance of predicting the presentation of low birth weight. Multiple Logistic Regression is one of the most expressive and versatile statistical instruments available for data analysis in both clinical and epidemiology. Its origin dates to the sixties with the transcendent work of Cornfield, Gordon & Smith on the risk of suffering from coronary heart disease and, in the way we know it today, with the contribution of Walter & Duncan in which addresses the issue of estimating the probability of occurrence of a certain event based on several variables. Its use has been universalized and expanded since the early eighties, due to the computer facilities available since then. Quantitative approach. The study design corresponds to that of an analytical observational epidemiological study of cases and controls with directionality response variable→explanatory variables and with prospective temporality. One thousand eight hundred fifteen newborns were studied [178 (9.81%) cases and 1,637 (90.19%) controls], which corresponds to nine controls per case. All term newborns (37 ≤ weeks of gestation ≤ 41) with weights < 2,500 g and ≥ 2,500 g were defined, respectively, as a case and as a control. The values obtained from the β Exponents or Odds Ratios indicate the positive contribution (OR> 1) in ascending numerical order of the explanatory variables alcoholism (0.0018); low socioeconomic level (0.5694); initiation of prenatal care from or after the 20th week of gestation (0.6116); birth interval ≤ 24 months (0.7942); age at menarche ≤ 12 years (1.0792); “unmarried” marital status (1.0961); female gender of the product (1.1271); maternal weight < 50 kg (1.4700); history of abortion(s) (1.5407); number of deliveries = 1 (1.5524); number of prenatal visits ≤ 5 (1.5966); type of delivery or abdominal birth route (1.6169); smoking (2.2019); number of deliveries ≥ 5 (2.2714); maternal age ≤ 19 years (2.4827); maternal age ≥ 36 years (2.8070); pathological obstetric history (4.0735); pathological personal history (4.6475); and maternal height < 150 cm (5.5092).
Objective. To determine if raw seafood, marinated without heat, partially cooked with heat, and completely cooked with heat that are sold for human consumption in establishments in the city of Telchac Puerto, Yucatan, Mexico, represent potential risk factors for the development of acute gastroenteritis, wound infection, primary septicemia and secondary septicemia by Vibrio vulnificus species. Material and methods. Study conducted on a representative sample (n= 132) selected from the total of 200 samples from 38 establishments. From July 1 to December 31, 2019, 132 samples of seafood were studied. Using the Cornfield method, the estimation interval was constructed at the 95% confidence level. Results. In 37 (28.03%) samples an equal number of strains were isolated whose biochemical characteristics corresponded to Vibrio vulnificus. The prevalences obtained in raw marine foods, marinated without heat, partially cooked with heat and completely cooked with heat were 35.59%, 45.45%, 22.45% and 0.00%. The Cornfield estimation interval at the 95% confidence level for Vibrio vulnificus was 13.56% ≤ P ≤ 42.50%. Conclusion. Raw seafood, marinated without heat, and partially cooked with heat represent potential risk factors for Vibrio vulnificus for the development of acute gastroenteritis, wound infection, primary septicemia, and secondary septicemia.
Introducción. Los caldos de enriquecimiento son útiles para la recuperación de Salmonella a partir de portadores, en los cuales el número de microorganismos puede ser tan bajo como 200 UFC/g de heces. En las técnicas de aislamiento de salmonela en microbiología sanitaria, la incubación de los caldos de enriquecimiento a 42°C es utilizada para mejorar la recuperación de la bacteria cuando se encuentra en muy pequeñas cantidades o cuando tiene que aislarse a partir de medios muy contaminados. Material y Métodos. Se estudiaron 2,080 muestras de heces de niños sin diarrea. Se inocularon caldos selenito de sodio y tetrationato de sodio, y ambos se incubaron a 37 y 42°C , y placas de agar XLD para aislamiento directo. Resultados. Ochenta y siete muestras (4.2%) fueron positivas al aislamiento de Salmonellaz spp. Cincuenta y dos muestras (2.5%) fueron positivas con selenito de sodio incubado a 37°C, y 54 (2.6%) a 42°C. El total de muestras positivas con el caldo selenito de sodio fue de 57 (2.7%). Setenta y una muestras (3.4%) fueron positivas con caldo tetrationato de sodio a 37°C y 73 (3.5%) a 42°C. El total de muestras positivas con caldo tetrationato de sodio fue de 76 (3.7%). En la siembra directa en agar XLD se aisló Salmonella en 9 (0.4%) muestras. Discusión. Encontramos que como medio de enriquecimiento el caldo tetrationato de sodio es mejor que el caldo selenito de sodio, y lo recomendamos para el trabajo clínico de rutina. Por otro lado, si es necesario llevar a cabo un estudio epidemiológico para detectar portadores, puede ser útil añadir el caldo selenito de sodio y utilizar dos temperaturas para el aislamiento Salmonella.
Introduction: Vibrio parahaemolyticus is a human pathogen that is widely distributed in marine environments. This organism is frequently isolated from a variety of raw seafood products, particularly fish and shellfish. Consumption of raw or undercooked fish and shellfish contaminated with Vibrio parahaemolyticus can lead to the development of acute gastroenteritis characterized by diarrhea, headache, vomiting, nausea, and abdominal cramps. It has also been isolated from wound infections and septicemias. Most cases are non–fatal. This bacterium is recognized as the leading cause of human gastroenteritis associated with fish and shellfish consumption in the United States of America and an important fish and shellfish borne pathogen worldwide. Chile faced its first outbreak in 1997–1998. Subsequently, outbreaks and cases have continued to occur, all associated with the consumption of fish and shellfish. Objective: To determine if raw seafood, marinated without heat, partially cooked with heat and completely cooked with heat that are sold for human consumption in establishments in the port of Chicxulub, Yucatan, Mexico, represent potential risk factors for the development of acute gastroenteritis, wound infection, primary septicemia and secondary septicemia by Vibrio parahaemolyticus species. Material and methods: Study conducted on a representative sample selected from the total of two hundred samples from thirty–eight establishments. From July 1 to December 31, 2021, one hundred thirty two samples of seafood were studied. Using the Cornfield Method, the estimation interval was constructed at the 95% confidence level. Results: In thirty–seven (28.03%) samples an equal number of strains were isolated whose biochemical characteristics corresponded to Vibrio parahaemolyticus. The prevalences obtained in raw marine foods, marinated without heat, partially cooked with heat and completely cooked with heat were 35.59%, 45.45%, 22.45% and 0.00%. The Cornfield estimation interval at the 95% confidence level for Vibrio parahaemolyticus was 13.56% ≤ P ≤ 42.50%. Conclusion: Raw seafood, marinated without heat and partially cooked with heat represent potential risk factors for Vibrio parahaemolyticus for the development of acute gastroenteritis, wound infection, primary septicemia, and secondary septicemia.
To determine if the marine foods of animal origin that are sold in establishments in the port of Chabihau, Yucatan, Mexico, represent potential risk factors for the Vibrio alginolyticus species for the development of acute gastroenteritis, wound infection, ear infection and primary septicemia. Three hundred and ninety seafood samples were studied: [298 (76.41%) raw, 8 (2.05%) marinated without heat, 77 (19.74%) partially cooked with heat and 7 (1.79%) completely cooked with heat]. For the homogenization and enrichment of the samples, as well as for the isolation and identification of the Vibrio alginolyticus species, we proceeded according to the methodology described in the eighth edition of the Bacteriological Analytical Manual (FDA). The criteria used in conducting the hypothesis tests for the difference between two percentages was based on the recommendations made by Cochran. The prevalences obtained in raw seafood, marinated without heat, partially cooked with heat and completely cooked with heat were, respectively, 7.72% (23/298), 0.00% (0/8), 7.79% (6/77) and 0.00% (0/7). In 29 (7.44%) samples an equal number of strains were isolated whose biochemical characteristics corresponded to the Vibrio alginolyticus species. It is concluded that raw seafood and partially cooked seafood represent potential risk factors for the Vibrio alginolyticus species for the development of acute gastroenteritis, wound infection, ear infection and primary septicemia.
The objective of this study was to statistically compare –using the One–Way Analysis of Variance and Dunnett's multiple comparisons test– the efficacy of Cyamopsis tetragonolobus L. and Cinnamomum verum versus the efficacy of the allopathic treatment Sil–Norboral (Glibenclamide 5 mg/Metformin 1000 mg) + Janumet (Sitagliptin 50 mg/Metformin 850 mg) as hypoglycemic agents in patients with type 2 diabetes mellitus who attend the "Dr. Agustin O'Horán" General Hospital for medical care. The epistemological approach is quantitative, probabilistic or positivistic. The study design corresponds to that of a therapeutic experimental epidemiological study (therapeutic clinical trial) with prospective temporality. Forty–eight patients with type 2 diabetes mellitus were studied. The 48 patients were randomly assigned to three groups: two experimental groups and one control group. Each group was made up of 16 patients. The first experimental group was given Cyamopsis tetragonolobus L. (twelve dry leaves of Cyamopsis tetragonolobus L. and 500 ml of hot water); pour the 500 ml of hot water into a container and add the twelve dry leaves of Cyamopsis tetragonolobus L.; take 250 ml in the morning and 250 ml at night); the second experimental group was given Cinnamomum verum (two g of dry leaves of Cinnamomum verum and 500 ml of hot water; pour the 500 ml of hot water into a container and add two g of dry leaves of Cinnamomum verum; take 250 ml in the morning and 250 ml at night); and the control group was given the Sil–Norboral allopathic treatment (Glibenclamide 5 mg/Metformin 1000 mg) + Janumet (Sitagliptin 50 mg/Metformin 850 mg). In ascending numerical order, the arithmetic means of the hematic glucose values corresponded to the following treatments: Cyamopsis tetragonolobus L. (84.06 mg/100mL); Cinnamomum verum (88.63mg/100mL); and Sil–Norboral (Glibenclamide 5 mg/Metformin 1000 mg) + Janumet (Sitagliptin 50 mg/Metformin 850 mg) (122.25 mg/100mL). The One–Way Analysis of Variance reported a value of F= 56.03 with a value of p= 0.0000, which indicates a statistically significant difference between a pair or between more than one pair of arithmetic means. Dunnett's multiple comparisons test showed statistically significant differences between the treatment with the medicinal plant Cyamopsis tetragonolobus L. and the allopathic treatment Sil–Norboral + Janumet: p= 0000. Likewise, Dunnett's multiple comparisons test showed a statistically significant difference between the treatment with the medicinal plant Cinnamomum verum and the allopathic treatment Sil–Norboral + Janumet: p= 0.0000. It is concluded that Cyamopsis tetragonolobus L. is, from a numerical and not a statistical point of view, the best hypoglycemic agent for the treatment of type 2 diabetes mellitus.
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