The demand for metallic nanoparticles synthesized using green methods has increased due to their various therapeutic and clinical applications, and plant biotechnology may be a potential resource facilitating sustainable methods of AgNPs synthesis. In this study, we evaluate the capacity of extracts from Randia aculeata cell suspension culture (CSC) in the synthesis of AgNPs at different pH values, and their activity against pathogenic bacteria and cancer cells was evaluated. Using aqueous CSC extracts, AgNPs were synthesized with 10% (w/v) of fresh biomass and AgNO3 (1 mM) at a ratio of 1:1 for 24 h of incubation and constant agitation. UV-vis analysis showed a high concentration of AgNPs as the pH increased, and TEM analysis showed polydisperse nanoparticles with sizes from 10 to 90 nm. Moreover, CSC extracts produce reducing agents such as phenolic compounds (162.2 ± 27.9 mg gallic acid equivalent/100 g biomass) and flavonoids (122.07 ± 8.2 mg quercetin equivalent/100 g biomass). Notably, AgNPs had strong activity against E. coli, S. pyogenes, P. aeruginosa, S. aureus, and S. typhimurium, mainly with AgNPs at pH 6 (MIC: 1.6 to 3.9 µg/mL). AgNPs at pH 6 and 10 had a high antiproliferative effect on cancer cells (IC50 < 5.7 µg/mL). Therefore, the use of cell suspension cultures may be a sustainable option for the green synthesis of AgNPs.
This study examines the effect of particle size and wood flour content on the properties of polystyrene filled with white oak flour. Wood-plastic ratios 10:90, 30:70 and 50:50% (wt/wt) and particle size 40, 50, 65 and 100 mesh were used. Tensile, bending and impact bending strength as well as the melt flow index were evaluated. Additionally, composite density and water absorption capacity were also tested. Scanning electronic microscopy revealed good adhesion between wood particles and polystyrene. Results show that mechanical properties are strongly influenced by wood flour content and particle size. A reduction in tensile module, elongation and deflection were observed, however, the bending module was increased. Impact strength increased with particle size and content. Melt flow index values are reduced with the increasing amount of filler content while water absorption increases with the amount of wood particles.
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