Myofibroblastic tumor (MIT) is characterized by the infiltration of different organs, most commonly the lungs, with nodular lesions composed of myofibroblasts and inflammatory cells, which can be identified by specific patterns in the immunohistochemical studies. When it involves the peritoneum it is difficult to eradicate, tends to relapse and it has an invasive behavior, requiring its differentiation from peritoneal carcinomatosis. Treatment may be surgical excision, the use of non-steroidal anti-inflammatory drugs (NSAIDs) and glucocorticoids. We report a 30 years old female with an unremarkable medical history, presenting with abdominal pain and progressive abdominal distention. A CT scan revealed multiple peritoneal nodular lesions. A surgical biopsy was reported as a myofibroblast and inflammatory cell infiltrate. Immunohistochemical staining was consistent with MIT. Given the extensive involvement of the peritoneum surgical therapy was not considered appropriate and treatment with NSAID and glucocorticoids was started. No response was observed after 6 months, therefore infliximab therapy was started. After 10 months of follow-up the patient is well, returned to normal life, ascites improved and resolved and CT scan showed partial regression or stabilization of the lesions.
Introduction 3. Materials 3.1 Materials 3.2 Cell Culture 3.3 Analysis of cell number, size and intracellular amino acids 3.4 SDS-PAGE and immunoblotting 3.5 Measurement of System A uptake 3.6 Proteomic analysis of TAP-tagged SNAT2 complexes 3.7 Statistical analysis 4. Results 4.1 MCF-7 cells express SNAT1, SNAT2 and exhibit functional System A transport 4.2 Effects of sustained System A inhibition on cell growth and proliferation 4.3 Chronic cell incubation with Me-AIB and intracellular amino acid levels 4.4 System A-induced modulation of mTOR signalling 4.5 Analysis of TAP-tagged SNAT2 protein complexes 5. Discussion 6. Acknowledgment 7. References Abbreviations: Methyl aminoisobutyrate, Me-AIB; mTORC, mammalian target of rapamycin complex; TAP, tandem affinity purification; MLCK, myosin light chain kinase; BCAA, branched chain amino acids.
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